首页 > 过刊浏览>2023年第60卷第6期 >1790-1800. DOI:10.11766/trxb202205150255
PDF HTML阅读 XML下载 导出引用 引用提醒
稻虾种养新模式对稻田土壤肥力和微生物群落结构的影响
作者:
中图分类号:

S154.3

基金项目:

国家自然科学基金项目（42167006、41967055）、江西省水利科学院江西省鄱阳湖水资源与环境重点实验室开放研究基金项目（2020GPSYS03）和江西省自然科学基金项目（20212BAB203023）资助


Effects of a New Rice-Shrimp Farming Model on Soil Fertility and Microbial Community Structure in Paddy Field
Author:
  • LAI Zheng

    LAI Zheng

    College of Geography and Environment, Jiangxi Normal University, Nanchang 330022, China;Jiangxi Provincial Key Laboratory of Protection and Utilization of Subtropical Plant Resources, College of Life Science, Jiangxi Normal University, Nanchang 330022, China;Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education, Jiangxi Normal University, Nanchang 330022, China;Foshan University, Foshan, Guangdong 528225, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • XIAO Liting

    XIAO Liting

    Jiangxi Provincial Key Laboratory of Protection and Utilization of Subtropical Plant Resources, College of Life Science, Jiangxi Normal University, Nanchang 330022, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • LAI Sheng

    LAI Sheng

    Jiangxi Provincial Key Laboratory of Protection and Utilization of Subtropical Plant Resources, College of Life Science, Jiangxi Normal University, Nanchang 330022, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • YANG Huilin

    YANG Huilin

    Jiangxi Provincial Key Laboratory of Protection and Utilization of Subtropical Plant Resources, College of Life Science, Jiangxi Normal University, Nanchang 330022, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • NI Caiying

    NI Caiying

    College of Geography and Environment, Jiangxi Normal University, Nanchang 330022, China;Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education, Jiangxi Normal University, Nanchang 330022, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • YANG Wenjing

    YANG Wenjing

    College of Geography and Environment, Jiangxi Normal University, Nanchang 330022, China;Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education, Jiangxi Normal University, Nanchang 330022, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • JIAN Minfei

    JIAN Minfei

    College of Geography and Environment, Jiangxi Normal University, Nanchang 330022, China;Jiangxi Provincial Key Laboratory of Protection and Utilization of Subtropical Plant Resources, College of Life Science, Jiangxi Normal University, Nanchang 330022, China;Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education, Jiangxi Normal University, Nanchang 330022, China
    在期刊界中查找
    在百度中查找
    在本站中查找
Fund Project:

Supported by the National Natural Science Foundation of China (No.42167006, 41967055), the Open Research Project of Key Laboratory of Poyang Lake Water Resources and Environment of Jiangxi Province in Jiangxi Academy of Water Science and Engineering (No. 2020GPSYS03) and Jiangxi Provincial Natural Science Foundation Project (20212BAB203023)

  • 摘要
    • | |
  • 访问统计
    • |
  • 参考文献 [37]
    • |
  • 相似文献 [20]
    • | | |
  • 文章评论
    摘要:

    为探究稻虾种养新模式对稻田土壤肥力和微生物群落组成的影响，基于稻田土壤理化指标测定和16S rDNA扩增子高通量测序技术，比较稻虾种养新模式（NRS）、稻虾种养传统模式（TRS）和水稻单作模式（CK）对稻田土壤理化指标和微生物多样性和群落结构的影响。结果显示：与CK和TRS相比，稻虾种养新模式能显著提高土壤pH、有机质、有效磷、速效钾含量和土壤微生物多样性和丰富度。与CK相比，NRS稻田土壤细菌优势菌门中的酸杆菌门、绿弯菌门和疣微菌门相对丰度分别增加30.89%、36.38%和2.16%，变形菌门和脱硫菌门相对丰度分别降低17.96%和58.59%。与TRS相比，NRS稻田土壤细菌优势菌门中的脱硫菌门相对丰度增加14.93%，绿弯菌门、疣微菌门、变形菌门和酸杆菌门相对丰度分别降低39.29%、37.42%、16.27%和6.81%。相关性分析表明，土壤有机质、速效氮、有效磷、速效钾和pH是影响稻田土壤微生物群落结构的主要理化因子。以上研究表明，稻虾种养新模式有利于提升稻田土壤肥力，显著提高了稻田土壤微生物多样性和改善微生物群落结构；研究结果对稻虾种养新模式的示范推广具有一定的指导意义和参考价值。

    Abstract:

    【Objective】This study was designed to explore the impact of a new rice-shrimp farming model (NRS) on soil fertility and microbial community composition in paddy field. 【Method】Three different farming treatments: new rice-shrimp farming model (NRS), traditional rice-shrimp farming model (TRS) and rice monoculture (CK) were set up, with CK as control. The paddy field physicochemical indexes and microbial diversity and community structure were determined by using the soil agrochemical analysis method and 16S rDNA amplicon high-throughput sequencing technology. 【Result】Compared with CK and TRS, NRS significantly improved the contents of soil organic matter, available K, available P and pH. NRS significantly increased the microbial diversity and species richness. A total of 32 phyla, 80 classes, 202 orders, 347 families and 491 genera of bacterial groups were detected in the paddy field of three models. The dominant bacterial phyla were Proteobacteria, Acidobacteriota, Chloroflexi, Verrucomicrobiota and Desulfobacterota. Compared with CK, NRS changed the composition of the soil microbial community. Among the bacterial dominant phyla, the relative abundances of Acidobacteria, Chloroflexi and Verrucomicrobiota were increased by 30.89%, 36.38% and 2.16%, while the relative abundance of Proteobacteria and Desulfobacteria decreased by 17.96% and 58.59%, respectively. Compared with TRS, NRS also changed the composition of the soil microbial community. Among the bacterial dominant phyla, the relative abundances of Desulfobacteria increased by 14.93%, while the relative abundance of Chloroflexi, Verrucomicrobiota, Proteobacteria and Acidobacteria decreased by 39.29%, 37.42%, 16.27% and 6.81%, respectively. Correlation analysis showed that soil organic matter, available potassium, available phosphorus, available nitrogen and pH were the main physicochemical factors affecting the structure of soil microbial community in paddy field. 【Conclusion】The new rice-shrimp farming model is conducive to improving soil fertility, significantly improving soil microbial diversity and altering microbial community structure. The results of this study have certain guiding significance and reference for the demonstration and promotion of the new rice-shrimp farming model.

    参考文献
    [1] Cao C G,Cai M L. Ecological agriculture model and technology of paddy planting and raising[M]. Beijing:Science Press,2017.[曹凑贵,蔡明历. 稻田种养生态农业模式与技术[M]. 北京:科学出版社,2017.]
    [2] Cai C,Li G,Zhu J Q,et al. Effects of rice-crawfish rotation on soil physicochemical properties in Jianghan Plain[J]. Acta Pedologica Sinica,2019,56(1):217-226.[蔡晨,李谷,朱建强,等. 稻虾轮作模式下江汉平原土壤理化性状特征研究[J]. 土壤学报,2019,56(1):217-226.]
    [3] Xiao F,Chen X,Cheng Y X. Model and case of integrated rice-fishing cultivation[M]. Beijing:China Agriculture Press,2019.[肖放,陈欣,成永旭. 稻渔综合种养技术模式与案例[M]. 北京:中国农业出版社,2019.]
    [4] Xu H,Zhao Y Y,Sun D Y,et al. Progress in integrated rice-crayfish farming system[J]. Journal of Agricultural Science and Technology,2022,24(2):160-168.[许辉,赵阳阳,孙东岳,等. 稻虾共作模式研究进展[J]. 中国农业科技导报,2022,24(2):160-168.]
    [5] National Fisheries Technology Extension Center,China Society of Fisheries. China crayfish industry development report(2021)[J]. China Fisheries,2021(7):27-33.[全国水产技术推广总站、中国水产学会. 中国小龙虾产业发展报告(2021)[J]. 中国水产,2021(7):27-33.]
    [6] Zhang J Z,Li Y Z,Li Y,et al. Advances in the indicator system and evaluation approaches of soil health[J]. Acta Pedologica Sinica,2022,59(6):603-616.[张江周,李奕赞,李颖,等. 土壤健康指标体系与评价方法研究进展[J]. 土壤学报,2022,59(6):603-616.]
    [7] Xiong S G,Lü Y Z,Cheng C Y. Soil fertility and its evaluation methods[J]. Journal of China Agricultural University,1997,2(S2):21-24.[熊顺贵,吕贻忠,成春彦. 土壤肥力概念与肥力评判方法[J]. 中国农业大学学报,1997,2(S2):21-24.]
    [8] Li X Z,Luo Y M,Hou D Y. The indicators,framework and procedures for soil health:A critical review[J]. Acta Pedologica Sinica,2022,59(6):617-624.[李烜桢,骆永明,侯德义. 土壤健康评估指标、框架及程序研究进展[J]. 土壤学报,2022,59(6):617-624.]
    [9] Li W B,Liu S J,Ye X X,et al. Effects of the co-culture of rice and aquatic animals on soil eco-system:A review[J]. Journal of Ecology and Rural Environment,2021,37(10):1292-1300.[李文博,刘少君,叶新新,等. 稻田综合种养模式对土壤生态系统的影响研究进展[J]. 生态与农村环境学报,2021,37(10):1292-1300.]
    [10] Si G H,Peng C L,Yuan J F,et al. Changes in soil microbial community composition and organic carbon fractions in an integrated rice-crayfish farming system in subtropical China[J]. Scientific Reports,2017,7:2856.
    [11] Si G H,Peng C L,Xu X Y,et al. Effect of integrated rice-crayfish farming system on soil physico-chemical properties in waterlogged paddy soils[J]. Chinese Journal of Eco-Agriculture,2017,25(1):61-68.[佀国涵,彭成林,徐祥玉,等. 稻虾共作模式对涝渍稻田土壤理化性状的影响[J]. 中国生态农业学报,2017,25(1):61-68.]
    [12] Wu B L,Chen G S,Zhao H M,et al. Effects of the integrated rice-crayfish mode on soil fertility in Chaohu Lake region[J]. Journal of Anhui Agricultural University,2018,45(1):96-100.[吴本丽,陈贵生,赵慧敏,等. 巢湖地区稻虾共作模式对稻田土壤肥力的影响[J]. 安徽农业大学学报,2018,45(1):96-100.]
    [13] Fuhrman J A. Microbial community structure and its functional implications[J]. Nature,2009,459(7244):193-199.
    [14] Hermans S M,Buckley H L,Case B S,et al. Using soil bacterial communities to predict physico-chemical variables and soil quality[J]. Microbiome,2020,8(1):79.
    [15] Hill G T,Mitkowski N A,Aldrich-Wolfe L,et al. Methods for assessing the composition and diversity of soil microbial communities[J]. Applied Soil Ecology,2000,15(1):25-36.
    [16] Acosta-Martínez V,Dowd S,Sun Y,et al. Tag-encoded pyrosequencing analysis of bacterial diversity in a single soil type as affected by management and land use[J]. Soil Biology & Biochemistry,2008,40(11):2762-2770.
    [17] Si G H,Peng C L,Xu X Y,et al. Effects of rice-crayfish integrated mode on soil microbial functional diversity and fertility in waterlogged paddy field[J]. Soils,2016,48(3):503-509.[佀国涵,彭成林,徐祥玉,等. 稻-虾共作模式对涝渍稻田土壤微生物群落多样性及土壤肥力的影响[J]. 土壤,2016,48(3):503-509.]
    [18] Zhu X X,Peng C L,Si G H,et al. Effect of rice-crayfish integrated system on soil bacterial community structure and diversity in paddy field[J]. Chinese Journal of Soil Science,2021,52(5):1121-1128.[朱秀秀,彭成林,佀国涵,等. 稻虾共作模式对稻田土壤细菌群落结构与多样性的影响[J]. 土壤通报,2021,52(5):1121-1128.]
    [19] Wang R,Zhu J,Jin T,et al. Characteristics of ammonia oxidation microbial abundance and community structure in paddy soils of rice-crayfish symbiosis farming system[J]. Journal of Plant Nutrition and Fertilizers,2019,25(11):1887-1899.[王蓉,朱杰,金涛,等. 稻虾共作模式下稻田土壤氨氧化微生物丰度和群落结构的特征[J]. 植物营养与肥料学报,2019,25(11):1887-1899.]
    [20] Bao S D. Soil and agricultural chemistry analysis[M]. 3rd ed. Beijing:China Agriculture Press,2000.[鲍士旦. 土壤农化分析[M]. 3版. 北京:中国农业出版社,2000.]
    [21] Dennis K L,Wang Y W,Blatner N R,et al. Adenomatous polyps are driven by microbe-instigated focal inflammation and are controlled by IL-10-producing T cells[J]. Cancer Research,2013,73(19):5905-5913.
    [22] Zhang Y J,Xu J,Lu R C,et al. Modification of quicklime on acid soil under forest and their effect on the growth of Panax notoginseng[J]. Chinese Journal of Applied Ecology,2022,33(4):972-980.[张义杰,徐杰,陆仁窗,等. 生石灰对林下酸化土壤的调控作用及三七生长的影响[J]. 应用生态学报,2022,33(4):972-980.]
    [23] Zhang L H,Su G X,Deng X H,et al. Effects of modifier application on nutrient contents of acid tobacco soil[J]. Chinese Tobacco Science,2020,41(5):20-27.[张龙辉,粟戈璇,邓小华,等. 改良剂施用对酸性植烟土壤养分的影响效应[J]. 中国烟草科学,2020,41(5):20-27.]
    [24] Si S C,Wu Y C,Li Y,et al. The Current research progress and prospects of cultivated and grassland soil health-A review[J]. Acta Pedologica Sinica,2022,59(6):626-641.[司绍诚,吴宇澄,李远,等. 耕地和草地土壤健康研究进展与展望[J]. 土壤学报,2022,59(6):626-641.]
    [25] Wang S G,Lin X G. Effect of mycorrhiza on bioremediation of polluted soil[J]. Rural Eco-Environment,2001,17(1):56-59.[王曙光,林先贵. 菌根在污染土壤生物修复中的作用[J]. 农村生态环境,2001,17(1):56-59.]
    [26] Chen L,Wan W T,Liu B,et al. Effects of rice-crayfish integrated system on microbial diversity and community structure in paddy water[J]. Journal of Huazhong Agricultural University,2022,41(1):141-151.[陈玲,万韦韬,刘兵,等. 稻虾共作对稻田水体微生物多样性和群落结构的影响[J]. 华中农业大学学报,2022,41(1):141-151.]
    [27] Zhu J,Liu H,Wu B K,et al. Effects of integrated rice-crayfish farming system on community structure and diversity of nirK denitrification microbe in paddy soils[J]. Chinese Journal of Eco-Agriculture,2018,26(9):1324-1332.[朱杰,刘海,吴邦魁,等. 稻虾共作对稻田土壤nirK反硝化微生物群落结构和多样性的影响[J]. 中国生态农业学报,2018,26(9):1324-1332.]
    [28] Wang F G,Song L,Feng Y,et al. Characteristics of soil microbiology in different planting-life orchard acid soils[J]. Chinese Journal of Soil Science,2011,42(1):46-50.[王富国,宋琳,冯艳,等. 不同种植年限酸化果园土壤微生物学性状的研究[J]. 土壤通报,2011,42(1):46-50.]
    [29] Song Y,Wang P,Wei Y P. Effects of different co-cultivation patterns of rice field on soil bacterial communities structure[J]. Acta Agriculturae Boreali- Occidentalis Sinica,2020,29(2):216-223.[宋宇,王鹏,韦月平. 不同稻田共作模式对土壤细菌群落结构的影响[J]. 西北农业学报,2020,29(2):216-223.]
    [30] Chaudhry V,Rehman A,Mishra A,et al. Changes in bacterial community structure of agricultural land due to long-term organic and chemical amendments[J]. Microbial Ecology,2012,64(2):450-460.
    [31] Wang P,Chen B,Zhang H. High throughput sequencing analysis of bacterial communities in soils of a typical Poyang Lake wetland[J]. Acta Ecologica Sinica,2017,37(5):1650-1658.[王鹏,陈波,张华. 基于高通量测序的鄱阳湖典型湿地土壤细菌群落特征分析[J]. 生态学报,2017,37(5):1650-1658.]
    [32] Muyzer G,Stams A J M. The ecology and biotechnology of sulphate-reducing bacteria[J]. Nature Reviews Microbiology,2008,6(6):441-454.
    [33] Langwig M V,de Anda V,Dombrowski N,et al. Large- scale protein level comparison of Deltaproteobacteria reveals cohesive metabolic groups[J]. The ISME Journal,2022,16(1):307-320.
    [34] Murphy C L,Biggerstaff J,Eichhorn A,et al. Genomic characterization of three novel Desulfobacterota classes expand the metabolic and phylogenetic diversity of the phylum[J]. Environmental Microbiology,2021,23(8):4326-4343.
    [35] Wang G H,Liu J J,Yu Z H,et al. Research progress of acidobacteria ecology in soils[J]. Biotechnology Bulletin,2016,32(2):14-20.[王光华,刘俊杰,于镇华,等. 土壤酸杆菌门细菌生态学研究进展[J]. 生物技术通报,2016,32(2):14-20.]
    [36] Xian W D,Zhang O,Li W J. Research status and prospect on bacterial phylum Chloroflexi[J]. Acta Microbiologica Sinica,2020,60(9):1801-1820.[鲜文东,张潇橦,李文均. 绿弯菌的研究现状及展望[J]. 微生物学报,2020,60(9):1801-1820.]
    [37] Li P,Wu J Q,Sha C Y,et al. Effects of manure and organic fertilizer application on soil microbial community diversity in paddy fields[J]. Environmental Science,2020,41(9):4262-4272.[理鹏,吴建强,沙晨燕,等. 粪肥和有机肥施用对稻田土壤微生物群落多样性影响[J]. 环境科学,2020,41(9):4262-4272.]
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

赖政,肖力婷,赖胜,杨慧林,倪才英,阳文静,简敏菲.稻虾种养新模式对稻田土壤肥力和微生物群落结构的影响[J].土壤学报,2023,60(6):1790-1800. DOI:10.11766/trxb202205150255 LAI Zheng, XIAO Liting, LAI Sheng, YANG Huilin, NI Caiying, YANG Wenjing, JIAN Minfei. Effects of a New Rice-Shrimp Farming Model on Soil Fertility and Microbial Community Structure in Paddy Field[J]. Acta Pedologica Sinica,2023,60(6):1790-1800.

复制
分享
文章指标
  • 点击次数:393
  • 下载次数: 1464
  • HTML阅读次数: 1015
  • 引用次数: 0
历史
  • 收稿日期:2022-05-15
  • 最后修改日期:2022-07-19
  • 录用日期:2022-09-22
  • 在线发布日期: 2022-10-09
  • 出版日期: 2023-11-28
文章二维码
今日访问量：730 您是第8256527 位访问者
通讯地址：江苏省南京市江宁区麒麟街道创优路298号 邮政编码：211135 电话：025-86881237
网址：http://pedologica.issas.ac.cn/ E-mail：actapedo@issas.ac.cn
版权所有：《土壤学报》编辑部 技术支持：北京勤云科技发展有限公司 ICP:05004320号-1