首页 > 过刊浏览>2024年第61卷第1期 >223-234. DOI:10.11766/trxb202206210271
PDF HTML阅读 XML下载 导出引用 引用提醒
林分改变驱动的优势菌根真菌类型变化影响土壤有机碳积累
作者:
中图分类号:

S714.3

基金项目:

国家自然科学基金项目（31971631，41977083）资助


The Change in Dominant Mycorrhizal Fungi Type Induced by Stand Transformation Affects Soil Organic Carbon Accumulation
Author:
Fund Project:

Supported by the National Natural Science Foundation of China (Nos. 31971631 and 41977083)

  • 摘要
    • | |
  • 访问统计
    • |
  • 参考文献 [36]
    • |
  • 相似文献
    • | | |
  • 文章评论
    摘要:

    马尾松林（Pinus massoniana Lamb.）是典型的外生菌根（ectomycorrhiza，ECM）优势林，但是近年来受到松材线虫病的影响，生态服务功能下降，逐渐被丛枝菌根（arbuscular mycorrhiza，AM）占优势的阔叶林所替代，但亚热带地区马尾松林转变为阔叶林过程中，优势菌根类型的改变对土壤有机碳积累的影响仍不清楚。以建德市马尾松林和阔叶林为研究对象，通过高效液相色谱和中性脂肪酸、磷脂脂肪酸等技术，测定优势菌根真菌生物量、球囊霉素相关土壤蛋白（GRSP）含量以及土壤胞外酶活性和微生物群落特征。结果表明：AM真菌占优势的阔叶林（AMD）取代ECM真菌占优势的马尾松林（ECMD），土壤有机碳显著提高了36.81%，微生物碳利用效率（CUE）显著提高了53.85%，AM真菌生物量提高了25.57%，ECM真菌生物量下降45.04%，并且ECM真菌占优势的马尾松林受到更严重的氮限制。磷脂脂肪酸分析显示，相比于AM真菌占优势的阔叶林，ECM真菌占优势的马尾松林革兰氏阳性细菌（G+）以及革兰氏阳性与阴性细菌之比（G+/G-）分别显著下降了21.47%和6.46%。冗余分析（redundancy analysis，RDA）结果表明，AM真菌占优势和ECM真菌占优势的森林之间土壤微生物群落结构存在显著差异（P<0.05），其中AM真菌生物量和土壤有机碳与微生物群落结构变异显著相关。GRSP含量下降以及不同类型菌根真菌招募微生物类群不同是导致ECM真菌占优势森林土壤有机碳下降的重要原因。因此，亚热带地区马尾松林被阔叶林替代后增加森林土壤有机碳含量，提高森林碳汇功能。

    Abstract:

    【Objective】Masson pine forest (Pinus massoniana Lamb.) is a typical ectomycorrhizal (ECM) dominant forest. However, in recent years, the ecological service function of the Masson pine forest decreased due to pine wood nematode disease, and the Masson pine forest was gradually replaced by an Arbuscular mycorrhizal (AM) dominant broadleaved forest. However, it remains unclear what influence could be exerted by the changes of dominant mycorrhizal types on soil organic carbon accumulation during the conversion of Masson pine forest to broadleaf forest in the subtropical region. 【Method】In this study, the biomass of ECM fungi and AM fungi were determined by high-performance liquid chromatography (HPLC) and neutral lipid fatty acids (NLFA), respectively. At the same time, phospholipid fatty acids (PLFAs) technology was used to study the characteristics of the microbial community. The content of glomalin-related soil protein (GRSP) and the activities of soil extracellular enzymes was also determined in Masson pine and broadleaved forests in Jiande County, Zhejiang Province.【Result】The results showed that: AM fungi-dominated (AMD) broadleaved forest replaced ECM fungi-dominated (ECMD) Masson pine forest, soil organic carbon in AM fungi dominated broadleaved forest was significantly enhanced by 36.81%, microbial carbon use efficiency (CUE) significantly increased by 53.85%, and AM fungal biomass significantly increased by 25.57%. Moreover, compared with ECM fungi-dominated forests, the biomass of ECM fungi in AM fungi-dominated forests decreased significantly by 45.04%. The Masson pine forest, which was dominated by ECM fungi, was subjected to more severe microbial nitrogen limitation. Phospholipid fatty acids analysis showed that the gram-positive bacteria (G+) and the ratio of gram-positive bacteria to gram-negative bacteria (G+/G-) in Masson pine forest dominated by ECM fungi compared with the broadleaved forest dominated by AM fungi were significantly decreased by 21.47% and 6.46%, respectively. Redundancy analysis (RDA) results showed that there were significant differences in microbial community structure between forests dominated by AM fungi and ECM fungi (P<0.05), in which AM fungal biomass (R2=0.48, P=0.002) and soil organic carbon content (R2=0.47, P=0.003) were significantly correlated with the variation of microbial community structure (P<0.05).【Conclusion】The decrease of GRSP and the different recruit of microbial groups by different mycorrhizal fungi types were important reasons for the reduction of soil organic carbon content in forests dominated by ECM fungi compared to AM fungi dominated forests. Therefore, the substitution of broadleaved forest for Masson pine forest in the subtropical region increased the content of forest soil organic carbon and improved the function of the forest carbon sink.

    参考文献
    [1] Guo L D，Tian C J. Progress of the function of mycorrhizal fungi in the cycle of carbon and nitrogen[J]. Microbiology China，2013，40（1）：158-171. [郭良栋，田春杰. 菌根真菌的碳氮循环功能研究进展[J]. 微生物学通报，2013，40（1）：158-171.]
    [2] Anthony M A，Crowther T W，van der Linde S，et al. Forest tree growth is linked to mycorrhizal fungal composition and function across Europe[J]. The ISME Journal，2022，16（5）：1327-1336.
    [3] Ge J F，Yang W Z，Gao X F，et al. Impacts of mycorrhiza symbionts on ammonia-oxidizing microorganisms as affected by management intensity of Phyllostachys pubescens forests[J]. Acta Pedologica Sinica，2021，58（2）：505-513. [葛江飞，杨为中，高雄飞，等. 不同经营强度下毛竹丛枝菌根共生体对氨氧化微生物的影响[J]. 土壤学报，2021，58（2）：505-513.]
    [4] Yang Y R，Ding J H，Zhang T L，et al. Phenolic acids detection in soil of Pinus massoniana forest and its effect on shrub and grass germination[J]. Soils，2013，45（2）：291-294. [杨奕如，丁健桦，张桃林，等. 马尾松林土壤酚酸类物质检测及其对灌草萌发的影响[J]. 土壤，2013，45（2）：291-294.]
    [5] Cheeke T E，Phillips R P，Brzostek E R，et al. Dominant mycorrhizal association of trees alters carbon and nutrient cycling by selecting for microbial groups with distinct enzyme function[J]. New Phytologist，2017，214（1）：432-442.
    [6] Mei K C，Chen Y M，Fan Y X，et al. Effects of litters and phosphorus addition on soil carbon priming effect in Pinus massoniana forest[J]. Acta Pedologica Sinica，2022，59（4）：1089-1099. [梅孔灿，陈岳民，范跃新，等. 凋落叶和磷添加对马尾松林土壤碳激发效应的影响[J]. 土壤学报，2022，59（4）：1089-1099.]
    [7] Neumann J，Matzner E. Biomass of extramatrical ectomycorrhizal mycelium and fine roots in a young Norway spruce stand-a study using ingrowth bags with different substrates[J]. Plant and Soil，2013，371（1/2）：435-446.
    [8] Högberg M N，Högberg P. Extramatrical ectomycorrhizal mycelium contributes one-third of microbial biomass and produces，together with associated roots，half the dissolved organic carbon in a forest soil[J]. New Phytologist，2002，154（3）：791-795.
    [9] Soudzilovskaia N A，van der Heijden M G，Cornelissen J H，et al. Quantitative assessment of the differential impacts of arbuscular and ectomycorrhiza on soil carbon cycling[J]. New Phytologist，2015，208（1）：280-293.
    [10] Staddon P L，Ramsey C B，Ostle N，et al. Rapid turnover of hyphae of mycorrhizal fungi determined by AMS microanalysis of 14C[J]. Science，2003，300（5622）：1138-1140.
    [11] Jin W H，Shao S，Chen J H，et al. Research progress in the impact of different mycorrhizal types on soil carbon cycling[J]. Journal of Zhejiang A&F University，2021，38（5）：953-962. [金文豪，邵帅，陈俊辉，等. 不同类型菌根对土壤碳循环的影响差异研究进展[J]. 浙江农林大学学报，2021，38（5）：953-962.]
    [12] Carrara J E，Walter C A，Freedman Z B，et al. Differences in microbial community response to nitrogen fertilization result in unique enzyme shifts between arbuscular and ectomycorrhizal-dominated soils[J]. Global Change Biology，2021，27（10）：2049-2060.
    [13] Lu R K. Analytical methods for soil and agro-chemistry[M]. Beijing：China Agricultural Science and Technology Press，2000. [鲁如坤. 土壤农业化学分析方法[M]. 北京：中国农业科技出版社，2000.]
    [14] Vance E D，Brookes P C，Jenkinson D S. An extraction method for measuring soil microbial biomass C[J]. Soil Biology and Biochemistry，1987，19（6）：703-707.
    [15] Chen J H，Chen D，Xu Q F，et al. Organic carbon quality，composition of main microbial groups，enzyme activities，and temperature sensitivity of soil respiration of an acid paddy soil treated with biochar[J]. Biology and Fertility of Soils，2019，55（2）：185-197.
    [16] Zhao Y Z，Liang C F，Shao S，et al. Linkages of litter and soil C：N：P stoichiometry with soil microbial resource limitation and community structure in a subtropical broadleaf forest invaded by Moso bamboo[J]. Plant and Soil，2021，465（1/2）：473-490.
    [17] Que H，Ge Y Y，Kang F X，et al. Content and distribution of glomalin-related soil protein in soils of Nanjing under different land use types[J]. Soils，2015，47（4）：719-724. [阙弘，葛阳洋，康福星，等. 南京典型利用方式土壤中球囊霉素含量及剖面分布特征[J]. 土壤，2015，47（4）：719-724.]
    [18] Awad A，Majcherczyk A，Schall P，et al. Ectomycorrhizal and saprotrophic soil fungal biomass are driven by different factors and vary among broadleaf and coniferous temperate forests[J]. Soil Biology and Biochemistry，2019，131：9-18.
    [19] Cao M，Li Y，Sun Z X，et al. A new QuEChERS-HPLC method for determining ergosterol in soil[J]. Chinese Journal of Analysis Laboratory，2019，38（2）：162-166. [曹梦，李勇，孙忠祥，等. QuEChERS-HPLC法测定土壤中麦角甾醇含量[J]. 分析试验室，2019，38（2）：162-166.]
    [20] Qin H，Niu L M，Wu Q F，et al. Bamboo forest expansion increases soil organic carbon through its effect on soil arbuscular mycorrhizal fungal community and abundance[J]. Plant and Soil，2017，420（1/2）：407-421.
    [21] Zak D R，Pregitzer K S，Curtis P S，et al. Atmospheric CO2 and the composition and function of soil microbial communities[J]. Ecological Applications，2000，10（1）：47-59.
    [22] Olsson P A，Bååth E，Jakobsen I，et al. The use of phospholipid and neutral lipid fatty acids to estimate biomass of arbuscular mycorrhizal fungi in soil[J]. Mycological Research，1995，99（5）：623-629.
    [23] Barceló M，Bodegom P M，Tedersoo L，et al. Mycorrhizal tree impacts on topsoil biogeochemical properties in tropical forests[J]. Journal of Ecology，2022，110（6）：1271-1282.
    [24] Li J W，Shangguan Z P，Deng L. Variations of belowground C and N cycling between arbuscular mycorrhizal and ectomycorrhizal forests across China[J]. Soil Research，2020，58（5）：441-451.
    [25] Lin G，McCormack M L，Ma C，et al. Similar below- ground carbon cycling dynamics but contrasting modes of nitrogen cycling between arbuscular mycorrhizal and ectomycorrhizal forests[J]. New Phytologist，2017，213（3）：1440-1451.
    [26] Lindahl B D，Kyaschenko J，Varenius K，et al. A group of ectomycorrhizal fungi restricts organic matter accumulation in boreal forest[J]. Ecology Letters，2021，24（7）：1341-1351.
    [27] Averill C，Turner B L，Finzi A C. Mycorrhiza-mediated competition between plants and decomposers drives soil carbon storage[J]. Nature，2014，505（7484）：543-545.
    [28] Koven C D，Hugelius G，Lawrence D M，et al. Higher climatological temperature sensitivity of soil carbon in cold than warm climates[J]. Nature Climate Change，2017，7（11）：817-822.
    [29] Fu X，Song Q，Li S，et al. Dynamic changes in bacterial community structure are associated with distinct priming effect patterns[J]. Soil Biology and Biochemistry，2022，169：108671.
    [30] Fanin N，Kardol P，Farrell M，et al. The ratio of Gram- positive to Gram-negative bacterial PLFA markers as an indicator of carbon availability in organic soils [J]. Soil Biology and Biochemistry，2019，128：111-114.
    [31] Mitchell P J，Simpson A J，Soong R，et al. Shifts in microbial community and water-extractable organic matter composition with biochar amendment in a temperate forest soil[J]. Soil Biology and Biochemistry，2015，81：244-254.
    [32] Chen L F，He Z B，Wu X R，et al. Linkages between soil respiration and microbial communities following afforestation of alpine grasslands in the northeastern Tibetan Plateau[J]. Applied Soil Ecology，2021，161：103882.
    [33] Bi Q F. Different fertilization modes and cultivation years affect soil phosphorus availability and carbon-nitrogen- phosphorus coupling transformations and their microbial mechanism[M].Hangzhou：Zhejiang University，2020. [毕庆芳. 施肥模式和耕作年限影响土壤磷有效性和碳氮磷耦合转化的微生物学机制[D]. 杭州：浙江大学，2020.]
    [34] Hagenbo A，Hadden D，Clemmensen K E，et al. Carbon use efficiency of mycorrhizal fungal mycelium increases during the growing season but decreases with forest age across a Pinus sylvestris chronosequence[J]. Journal of Ecology，2019，107（6）：2808-2822.
    [35] Thoms C，Gattinger A，Jacob M，et al. Direct and indirect effects of tree diversity drive soil microbial diversity in temperate deciduous forest[J]. Soil Biology and Biochemistry，2010，42（9）：1558-1565.
    [36] Zhou J C，Zhang L，Feng G，et al. Arbuscular mycorrhizal fungi have a greater role than root hairs of maize for priming the rhizosphere microbial community and enhancing rhizosphere organic P mineralization[J]. Soil Biology and Biochemistry，2022，171：108713.
    相似文献
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

屠嘉莹,金文豪,盛卫星,邢佳佳,彭丽媛,陈俊辉,徐秋芳,秦华.林分改变驱动的优势菌根真菌类型变化影响土壤有机碳积累[J].土壤学报,2024,61(1):223-234. DOI:10.11766/trxb202206210271 TU Jiaying, JIN Wenhao, SHENG Weixing, XING Jiajia, PENG Liyuan, CHEN Junhui, XU Qiufang, QIN Hua. The Change in Dominant Mycorrhizal Fungi Type Induced by Stand Transformation Affects Soil Organic Carbon Accumulation[J]. Acta Pedologica Sinica,2024,61(1):223-234.

复制
分享
文章指标
  • 点击次数:367
  • 下载次数: 1578
  • HTML阅读次数: 1001
  • 引用次数: 0
历史
  • 收稿日期:2022-06-21
  • 最后修改日期:2022-08-01
  • 录用日期:2022-09-19
  • 在线发布日期: 2022-09-21
  • 出版日期: 2024-01-15
文章二维码
今日访问量：7226 您是第8199594 位访问者
通讯地址：江苏省南京市江宁区麒麟街道创优路298号 邮政编码：211135 电话：025-86881237
网址：http://pedologica.issas.ac.cn/ E-mail：actapedo@issas.ac.cn
版权所有：《土壤学报》编辑部 技术支持：北京勤云科技发展有限公司 ICP:05004320号-1