【Objective】To clarify the effects of fertilizer and organic fertilizer combined with biochar on the forms of nutrients and nitrogen in rhizosphere / non rhizosphere soil, which is helpful for the efficient utilization and scientific management of farmland nitrogen .【Methods】Taking the pot experiment of lemon as the research object, this paper set up six treatments: no fertilizer (CK), chemical fertilizer (CF), manure (M), chemical fertilizer with biochar (CFB), manure with biochar (MB) and fresh manure with biochar (FMB). By measuring the changes of nutrient content in rhizosphere and non rhizosphere soil and the transformation of soil nitrogen storage forms, the effects of organic fertilizer combined with biochar on the forms of soil nutrients and nitrogen in rhizosphere and non rhizosphere were studied. 【Result】The results showed that compared with CK treatment, MB treatment increased non rhizosphere and Rhizosphere Soil pH by 0.32 and 0.28 units, and FMB treatment increased rhizosphere soil pH by 0.63 units; MB and FMB treatments increased the content of organic matter in rhizosphere soil by 25.37% and 84.88% respectively, and significantly increase the total nitrogen content of Rhizosphere Soil by 25.42% and 50.93%. Combined application of biochar (CFB, MB and FMB) could promote the transformation of soil non convertible nitrogen (NTF-N) to two more active nitrogen forms, iron manganese oxide bound nitrogen (IMOF-N) and organic sulfide bound nitrogen (OSF-N). IMOF-N and OSF-N accounted for 35.85%~61.72% and 26.65%~46.56% of convertible nitrogen, respectively, and were the main components of rhizosphere and non rhizosphere soil convertible nitrogen (TF-N). Therefore, manure combined with biochar is the most effective way to improve nutrients and regulate nitrogen transformation in rhizosphere and non rhizosphere soil.