Nanjing Agricultural University
National Natural Science Foundation of China (42107221) and the National Science Fund for Distinguished Young Scholars of China (41925029)
抗生素抗性基因（Antibiotic resistance genes，ARGs）的产生和传播对全球公共健康产生巨大威胁。土壤作为ARGs的重要储存库和介质，已引起众多学者广泛关注。为全面了解土壤ARGs相关领域的研究进展及热点，本研究利用可视化软件VOSviewer和CiteSpace，基于Web of Science数据库，对2013–2022年发表的土壤ARGs领域相关文献进行计量学分析。结果表明，土壤ARGs相关研究的发文数量和被引频次逐年上升。我国在土壤ARGs领域的发文量最高，占总发文量的61.40%，且与澳大利亚、美国等27个国家合作紧密。四环素和磺胺嘧啶为该领域重点关注的抗生素类型；大肠杆菌作为模式菌一直是土壤ARGs领域备受关注的微生物类型。不同时期土壤ARGs研究热点存在明显差异：初期关注的重点方向为ARGs的认识和定量，随后引起较多关注的是ARGs源解析及其与微生物的内在联系，而对ARGs传播扩散和归趋相关研究已成为现今科学家关注和研究的热点。
【Objective】The increasing emergence and spread of antibiotic resistance genes (ARGs) poses a great threat to global public health. Soil as an important repository and medium for ARGs has attracted extensive attention from many scholars. 【Method】In order to have a comprehensive understanding of the research progress and hotspots in the field of soil ARGs, the VOSviewer and CiteSpace software were used to perform a bibliometric analysis of the papers related to soil ARGs published from 2013 to 2022 in “Web of Science” database. More specifically, density visualization analysis, keyword emergence analysis, and contribution network analysis were performed to deeply discuss the types of publications, annual frequency of citations, country/region and institution of publications, keywords, research directions, and the hotspots and trends of research. 【Result】Our results demonstrated that the number and citation frequency of the publications related to soil ARGs increased year by year. The number of publications increased slowly in the first five years (2013–2017), while it rapidly increased in the last five years (2018–2022). Besides, the maximum citation frequency was 22123 times. China published the most papers in the field of soil ARGs, accounting for 61.40% of the total number of publications, and China cooperated closely with 27 countries, including Australia and the United States. Tetracycline and sulfadiazine were the two main types of antibiotics in the soil ARGs field, and Escherichia coli as model bacterium was a microbial type of great interest in this field. The results of citation burst and co-occurrence analysis indicated that research hotspots of soil ARGs differed significantly in different periods. At first, scholars focused on the understanding and quantification of ARGs. Subsequently, more attention was paid to the source analysis of ARGs and their intrinsic connection with microorganisms. The study of the spread and fate of ARGs has become a hot topic of interest and research for scientists today. 【Conclusion】In conclusion, this study conducted a comprehensive bibliometric analysis of the relevant literature in the field of soil ARGs. On the whole, the research on the propagation deterrence and reduction techniques of soil ARGs is still insufficient. Future research may focus on the development and improvement of monitoring system as well as mathematical model establishment to assess the environmental risks of soil ARGs and their effects on human health. Besides, prevention-oriented source control of soil ARGs should be expanded. In addition, the diffusion mechanisms and influencing factors should be explored further to lay a solid foundation for ARGs reduction technology.
Zhang Youai, Wang Tingting, Hu Xiaojie, Qin Chao, Gao Yanzheng. Research Progress and Hotspots Analysis of Soil Antibiotic Resistance Genes[J]. Acta Pedologica Sinica,,[In Press]