两种母岩发育森林土壤微生物生物量碳代谢的差异性
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中图分类号:

S154.1

基金项目:

国家自然科学基金项目(U21A2007, U22A20560, 42107381)、广西优良用材林资源培育重点实验室课题项目(22-B-01-04)资助


Analysis of the Differences and Causes in Microbial Biomass Carbon Metabolism Characteristics of Forest Soils Developed from Two Types of Rocks
Author:
  • FU Ruitong

    FU Ruitong

    College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China;Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
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  • WAN Xiangyu

    WAN Xiangyu

    College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China;Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
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  • YANG Xinyi

    YANG Xinyi

    Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;Guangxi Key Laboratory of Karst Ecological Processes and Services, Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Science, Huanjiang 547100, Guangxi, China
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  • LI Dejun

    LI Dejun

    Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;Guangxi Key Laboratory of Karst Ecological Processes and Services, Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Science, Huanjiang 547100, Guangxi, China
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  • HU Peilei

    HU Peilei

    Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;Guangxi Key Laboratory of Karst Ecological Processes and Services, Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Science, Huanjiang 547100, Guangxi, China
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  • DUAN Pengpeng

    DUAN Pengpeng

    Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;Guangxi Key Laboratory of Karst Ecological Processes and Services, Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Science, Huanjiang 547100, Guangxi, China
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  • ZHANG Yuling

    ZHANG Yuling

    College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China
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Fund Project:

Supported by the National Natural Science Foundation of China (Nos. U21A2007, U22A20560, 42107381), Project funded by Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation (No.22-B-01-04)

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    摘要:

    针对不同母岩发育土壤的微生物生物量碳代谢特征及驱动因子不明确的科学问题,以石灰岩和碎屑岩两种母岩发育的森林土壤为研究对象,利用18O-H2O标记测定微生物生长速率、微生物呼吸速率、微生物生物量碳利用效率(CUE)以及微生物周转时间,并结合土壤理化性质、土壤有机质矿物保护特性和土壤酶活性以及微生物生物量和群落组成,明确岩性对森林土壤微生物生物量碳代谢的影响机制。结果表明:石灰岩发育土壤的pH和0.05mm~0.002 mm粒径含量高于碎屑岩发育土壤,而有机碳(SOC)、全氮(TN)、可溶性碳(DOC)、C:P和N:P却低于碎屑岩发育土壤(P<0.05);石灰岩发育土壤交换性钙镁(Ca+Mg)和游离态铁铝((Fe+Al)d)含量高于碎屑岩发育土壤,但非晶态铁铝((Fe+Al)o)含量则低于碎屑岩发育土壤;石灰岩发育土壤碳氮磷循环、相关酶活性均显著低于碎屑岩发育土壤(P<0.05);石灰岩发育土壤微生物生物量磷(MBP)高于碎屑岩发育土壤,但微生物生物量碳(MBC)、真菌细菌比(F:B)和革兰氏阳性菌阴性菌比(G+:G-)则显著低于碎屑岩发育土壤(P < 0.05);石灰岩发育土壤微生物生长速率和周转速率显著高于碎屑岩发育土壤(P < 0.05),但微生物呼吸速率和CUE在两种土壤之间差异并不显著。土壤微生物生长速率和微生物周转速率均与土壤pH、(Ca+Mg):(Fe+Al)o、(Ca+Mg):SOC、(Fe+Al)d:SOC和革兰氏阴性细菌呈显著正相关(P<0.05),而与DOC、铁铝结合态有机碳、酶活性、MBC:MBN、F:B和G+:G-比呈显著负相关(P < 0.05)。此外,土壤CUE与MBC和MBC:MBN呈显著负相关(P < 0.05);微生物呼吸速率仅与酚氧化酶活性呈显著负相关(P<0.05)。两种岩石发育的森林土壤微生物生物量碳代谢受生物和非生物因素的控制,这一研究结果为解释不同母岩发育森林土壤有机碳库的差异提供参考。

    Abstract:

    【Objective】Microbial biomass carbon(C)metabolism is vital in the formation and stabilization of organic C in soil, constituting a critical parameter in the models of terrestrial ecosystems. Yet, the variances in the microbial C metabolism indices in soils developed from different lithological origins remain undefined. 【Method】To address the scientific gap in the characteristics and driving factors of microbial biomass C metabolism in soils developed from different rocks, we sampled forest soils developed from limestone and clastic rocks as research objects. Using 18O-H2O labeling, we measured the microbial growth rate, respiration rate, carbon use efficiency (CUE), and turnover time. Combined with soil physicochemical properties, soil organic matter mineral protection characteristics, soil enzyme activity, and microbial biomass and community composition, we clarified the influencing mechanism of lithology on forest soil microbial biomass C metabolism. 【Result】The findings indicate that the pH and the 0.002~0.05 mm particle content in limestone-derived soils surpass those in clastic rock-derived soils, whereas soil organic carbon (SOC), total nitrogen (TN), dissolved organic carbon(DOC), C: P and N: P ratios were lower in limestone-derived soils (P<0.05). The limestone-developed soils had a higher content of exchangeable calcium and magnesium (Ca/Mg) and free iron and aluminum ((Fe+Al)d) than the clastic rock-developed soils, but the content of amorphous iron and aluminum((Fe+Al)o)was lower than that in the clastic rock-developed soils. Furthermore, the enzyme activity related to C, N, and P cycling in limestone-developed soils was significantly lower than that in clastic rock-developed soils (P< 0.05). In addition, the microbial biomass phosphorus (MBP) in limestone-developed soils was higher than that in clastic rock-developed soils, but microbial biomass carbon(MBC), fungi: bacteria ratio (F: B), and Gram-positive to Gram-negative bacteria ratio (G+: G-)were significantly lower than those in clastic rock-developed soils (P<0.05). The microbial growth rate and turnover rate in limestone-derived soils were significantly higher than in clastic rock-derived soils (P<0.05), but there was no significant difference in the microbial respiration rate and CUE between the two types of soils. Correlation analysis revealed that the soil microbial growth rate and turnover rate were significantly positively correlated with soil pH, (Ca+Mg): (Fe+Al)o, (Ca+Mg): SOC, (Fe+Al)d: SOC, and Gram-negative bacteria(P<0.05), and significantly negatively related to DOC, organic C bound to iron and aluminum, enzyme activity, MBC: MBN, F: B, and G+: G- ratio(P<0.05). The soil CUE was significantly negatively correlated with MBC and MBC: MBN (P<0.05) while microbial respiration rate was only significantly negatively correlated with phenol oxidase activity (P<0.05). In summary, the higher pH, weaker amorphous iron-aluminum mineral protection, lower microbial resource limitation, and larger bacterial biomass (especially Gram-negative bacteria) in limestone-derived soils may lead to greater microbial motility in these soils and stronger substrate availability, resulting in larger microbial growth and turnover rates. However, there was no difference in the soil microbial biomass CUE between the two rock types, which may be due to the similar soil C: N ratio. 【Conclusion】The microbial biomass C metabolism of forest soils developed from two types of rocks is controlled by biological and non-biological factors. These research results provide a new mechanism for explaining the differences in organic carbon pools in forest soils developed from different rocks.

    参考文献
    [1] Karhu K,Alaei S,Li J,et al. Microbial carbon use efficiency and priming of soil organic matter mineralization by glucose additions in boreal forest soils with different C:N ratios[J]. Soil Biology and Biochemistry,2022,167:108615.
    [2] Gunina A,Kuzyakov Y. From energy to(soil organic)matter[J]. Global Change Biology,2022,28(7):2169-2182.
    [3] Camenzind T,Mason-Jones K,Mansour I,et al. Formation of necromass-derived soil organic carbon determined by microbial death pathways[J]. Nature Geoscience,2023,16(2):115-122.
    [4] Schimel J. Modeling ecosystem-scale carbon dynamics in soil:The microbial dimension[J]. Soil Biology and Biochemistry,2023,178:108948.
    [5] Peng T,Ma S L,Ma C X,et al. Effects of long-term monocropping on soil microbial metabolic activity and diversity in topsoil and subsoil horizons of Lycium barbarum fields[J]. Acta Prataculturae Sinica,2023,32(1):89-98. [彭彤,马少兰,马彩霞,等. 长期单作对枸杞园不同土层土壤微生物代谢活性和多样性的影响[J]. 草业学报,2023,32(1):89-98.]
    [6] Schimel J,Weintraub M N,Moorhead D. Estimating microbial carbon use efficiency in soil:Isotope-based and enzyme-based methods measure fundamentally different aspects of microbial resource use[J]. Soil Biology and Biochemistry,2022,169:108677.
    [7] Kästner M,Miltner A,Thiele-Bruhn S,et al. Microbial necromass in soils-Linking microbes to soil processes and carbon turnover[J]. Frontiers in Environmental Science,2021,9:756378.
    [8] Kallenbach C M,Frey S D,Grandy A S. Direct evidence for microbial-derived soil organic matter formation and its ecophysiological controls[J]. Nature Communications,2016,7(1):1-10.
    [9] Xu X F,Wang N N,Lipson D,et al. Microbial macroecology:In search of mechanisms governing microbial biogeographic patterns[J]. Global Ecology and Biogeography,2020,29(11):1870-1886.
    [10] Gavazov K,Canarini A,Jassey V E J,et al. Plant-microbial linkages underpin carbon sequestration in contrasting mountain tundra vegetation types[J]. Soil Biology and Biochemistry,2022,165:108530.
    [11] Silva-Sánchez A,Soares M,Rousk J. Testing the dependence of microbial growth and carbon use efficiency on nitrogen availability,pH,and organic matter quality[J]. Soil Biology and Biochemistry,2019,134:25-35.
    [12] Kleber M,Bourg I C,Coward E K,et al. Dynamic interactions at the mineral-organic matter interface[J]. Nature Reviews Earth & Environment,2021,2(6):402-421.
    [13] Wilhelm R C,Lynch L,Webster T M,et al. Susceptibility of new soil organic carbon to mineralization during dry-wet cycling in soils from contrasting ends of a precipitation gradient[J]. Soil Biology and Biochemistry,2022,169:108681.
    [14] Hartmann M,Six J. Soil structure and microbiome functions in agroecosystems[J]. Nature Reviews Earth & Environment,2023,4(1):4-18.
    [15] Sokol N W,Sanderman J,Bradford M A. Pathways of mineral-associated soil organic matter formation:Integrating the role of plant carbon source,chemistry,and point of entry[J]. Global Change Biology,2019,25(1):12-24.
    [16] Finley B K,Mau R L,Hayer M,et al. Soil minerals affect taxon-specific bacterial growth[J]. The ISME Journal,2022,16(5):1318-1326.
    [17] Wang C,Qu L R,Yang L M,et al. Large-scale importance of microbial carbon use efficiency and necromass to soil organic carbon[J]. Global Change Biology,2021,27(10):2039-2048.
    [18] Wang S J,Li R L,Sun C X,et al. How types of carbonate rock assemblages constrain the distribution of Karst rocky desertified land in Guizhou Province,PR China:Phenomena and mechanisms[J]. Land Degradation & Development,2004,15(2):123-131.
    [19] Hu P L,Zhang W,Chen H S,et al. Lithologic control of microbial-derived carbon in forest soils[J]. Soil Biology and Biochemistry,2022,167:108600.
    [20] Hahm W J,Riebe C S,Lukens C E,et al. Bedrock composition regulates mountain ecosystems and landscape evolution[J]. Proceedings of the National Academy of Sciences of the United States of America,2014,111(9):3338-3343.
    [21] Jiang Z H,Liu H Y,Wang H Y,et al. Bedrock geochemistry influences vegetation growth by regulating the regolith water holding capacity[J]. Nature Communications,2020,11(1):1-9.
    [22] Weemstra M,Peay K G,Davies S J,et al. Lithological constraints on resource economies shape the mycorrhizal composition of a Bornean rain forest[J]. New Phytologist,2020,228(1):253-268.
    [23] Possinger A R,Weiglein TL,Bowman M M,et al. Climate effects on subsoil carbon loss mediated by soil chemistry[J]. Environmental Science & Technology,2021,55(23):16224-16235.
    [24] Brandt L,Stache F,Poll C,et al. Mineral type and land-use intensity control composition and functions of microorganisms colonizing pristine minerals in grassland soils[J]. Soil Biology and Biochemistry,2023,182:109037.
    [25] Stone B W G,Dijkstra P,Finley B K,et al. Life history strategies among soil bacteria-Dichotomy for few,continuum for many[J]. The ISME Journal,2023,17(4):611-619.
    [26] Chen L Y,Liu L,Qin S Q,et al. Regulation of priming effect by soil organic matter stability over a broad geographic scale. Nature Communications,2019,10:5112.
    [27] Cotrufo M F,Ranalli M G,Haddix M L,et al. Soil carbon storage informed by particulate and mineral-associated organic matter[J]. Nature Geoscience,2019,12(12):989-994.
    [28] Wang S M,Jia Y F,Liu T,et al. Delineating the role of calcium in the large-scale distribution of metal-bound organic carbon in soils[J]. Geophysical Research Letters,2021,48(10):11-21.
    [29] Saiya-Cork K R,Sinsabaugh R L,Zak D R. The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil[J]. Soil Biology and Biochemistry,2002,34(9):1309-1315.
    [30] Bligh E G,Dyer W J. A rapid method of total lipid extraction and purification[J]. Canadian Journal of Biochemistry and Physiology,1959,37(8):911-917.
    [31] Guo J F,Yang Z J,Lin C F,et al. Conversion of a natural evergreen broadleaved forest into coniferous plantations in a subtropical area:Effects on composition of soil microbial communities and soil respiration[J]. Biology and Fertility of Soils,2016,52(6):799-809.
    [32] Zheng Q,Hu Y T,Zhang S S,et al. Growth explains microbial carbon use efficiency across soils differing in land use and geology[J]. Soil Biology and Biochemistry,2019,128:45-55.
    [33] Wilson S G,Dahlgren R A,Margenot A J,et al. Expanding the Paradigm:The influence of climate and lithology on soil phosphorus. Geoderma,2022,421:115809.
    [34] Zhu D N,Zou S Z,Zhou C S,et al. Desorption characteristics and hysteresis of adsorbed cadmium in calcareous soils on Karst area[J]. Environmental Chemistry,2016,35(7):1407-1414. [朱丹尼,邹胜章,周长松,等. 岩溶区石灰性土壤对Cd2+吸附的解吸特性及滞后效应[J]. 环境化学,2016,35(7):1407-1414.]
    [35] Schrumpf M,Kaiser K,Guggenberger G,et al. Storage and stability of organic carbon in soils as related to depth,occlusion within aggregates,and attachment to minerals[J]. Biogeosciences,2013,10(3):1675-1691.
    [36] Rowley M C,Grand S,Adatte T,et al. A cascading influence of calcium carbonate on the biogeochemistry and pedogenic trajectories of subalpine soils,Switzerland[J]. Geoderma,2020,361:114065.
    [37] Qin S Q,Kou D,Mao C,et al. Temperature sensitivity of permafrost carbon release mediated by mineral and microbial properties[J]. Science Advances,2021,7(32):11-21.
    [38] Ye C L,Chen D M,Hall S J,et al. Reconciling multiple impacts of nitrogen enrichment on soil carbon:Plant,microbial and geochemical controls[J]. Ecology Letters,2018,21(8):1162-1173.
    [39] Duan X,Li Z,Liu M,et al. Research progress on iron-mediated soil organic carbon fixation and mineralization[J]. Advances in Earth Science,2022,37(2):202-211. [段勋,李哲,刘淼,等. 铁介导的土壤有机碳固持和矿化研究进展[J]. 地球科学进展,2022,37(2):202-211.]
    [40] Wang W H,Yu Y N,Xie J Q,et al. Accumulation and stoichiometric characteristics of soil carbon,nitrogen and phosphorus in different afforestation models in south subtropical region of China[J]. Acta Ecologica Sinica,2023,43(5):1793-1803. [王薇菡,虞依娜,谢嘉淇,等. 中国南亚热带不同造林模式碳汇林土壤碳、氮、磷的积累及化学计量特征[J]. 生态学报,2023,43(5):1793-1803.]
    [41] [孙彩丽,王艺伟,王从军,等. 喀斯特山区土地利用方式转变对土壤酶活性及其化学计量特征的影响[J]. 生态学报,2021,41(10):4140-4149.]
    [42] Chen H,Li D J,Xiao K C,et al. Soil microbial processes and resource limitation in Karst and non-Karst forests[J]. Functional Ecology,2018,32(5):1400-1409.
    [43] Fanin N,Kardol P,Farrell M,et al. The ratio of Gram-positive to Gram-negative bacterial PLFA markers as an indicator of carbon availability in organic soils[J]. Soil Biology and Biochemistry,2019,128:111-114.
    [44] Islam M R,Singh B,Dijkstra F A. Microbial carbon use efficiency of glucose varies with soil clay content:A meta-analysis[J]. Applied Soil Ecology,2023,181:104636.
    [45] Jones D L,Cooledge E C,Hoyle F C,et al. pH and exchangeable aluminum are major regulators of microbial energy flow and carbon use efficiency in soil microbial communities[J]. Soil Biology and Biochemistry,2019,138:107584.
    [46] Mganga K Z,Sietio O M,Meyer N,et al. Microbial carbon use efficiency along an altitudinal gradient. Soil Biology and Biochemistry,2022,173:108799.
    [47] Pold G,Kwiatkowski B L,Rastetter E B,et al. Sporadic P limitation constrains microbial growth and facilitates SOM accumulation in the stoichiometrically coupled,acclimating microbe-plant-soil model[J]. Soil Biology and Biochemistry,2022,165:108489.
    [48] Yang T H,Li X J,Hu B,et al. Soil microbial biomass and community composition along a latitudinal gradient in the arid valleys of southwest China[J]. Geoderma,2022,413:115750.
    [49] Zheng H F,Vesterdal L,Schmidt I K,et al. Ecoenzymatic stoichiometry can reflect microbial resource limitation,substrate quality,or both in forest soils[J]. Soil Biology and Biochemistry,2022,167:108613.
    [50] Kamble P N,Bååth E. Comparison of fungal and bacterial growth after alleviating induced N-limitation in soil[J]. Soil Biology and Biochemistry,2016,103:97-105.
    [51] Nottingham A T,Hicks L C,Ccahuana A J Q,et al. Nutrient limitations to bacterial and fungal growth during cellulose decomposition in tropical forest soils[J]. Biology and Fertility of Soils,2018,54(2):219-228.
    [52] Feng X H,Qin S Q,Zhang D Y,et al. Nitrogen input enhances microbial carbon use efficiency by altering plant-microbe-mineral interactions[J]. Global Change Biology,2022,28(16):4845-4860.
    [53] Johnston E R,Kim M,Hatt J K,et al. Phosphate addition increases tropical forest soil respiration primarily by deconstraining microbial population growth[J]. Soil Biology and Biochemistry,2019,130:43-54.
    [54] Morris K A,Richter A,Migliavacca M,et al. Growth of soil microbes is not limited by the availability of nitrogen and phosphorus in a Mediterranean oak-savanna[J]. Soil Biology and Biochemistry,2022,169:108680.
    [55] Angst G,Pokorný J,Mueller C W,et al. Soil texture affects the coupling of litter decomposition and soil organic matter formation[J]. Soil Biology and Biochemistry,2021,159:108302.
    [56] Khan K S,Joergensen R G. Stoichiometry of the soil microbial biomass in response to amendments with varying C/N/P/S ratios[J]. Biology and Fertility of Soils,2019,55(3):265-274.
    [57] Sinsabaugh R L,Turner B L,Talbot J M,et al. Stoichiometry of microbial carbon use efficiency in soils[J]. Ecological Monographs,2016,86(2):172-189.
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付瑞桐,万翔宇,杨馨逸,李德军,胡培雷,段鹏鹏,张玉玲.两种母岩发育森林土壤微生物生物量碳代谢的差异性[J].土壤学报,2024,61(5):1432-1443. DOI:10.11766/trxb202302210071 FU Ruitong, WAN Xiangyu, YANG Xinyi, LI Dejun, HU Peilei, DUAN Pengpeng, ZHANG Yuling. Analysis of the Differences and Causes in Microbial Biomass Carbon Metabolism Characteristics of Forest Soils Developed from Two Types of Rocks[J]. Acta Pedologica Sinica,2024,61(5):1432-1443.

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  • 收稿日期:2023-02-21
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