长期秸秆掩埋配施氮肥对土壤细菌群落特征的影响
作者:
作者单位:

1.中国科学院南京土壤研究所;2.中国科学院封丘农业生态试验站;3.河南城建学院,测绘与城市空间信息学院

作者简介:

通讯作者:

中图分类号:

基金项目:

国家重点研发计划项目(2022YFD1500903,2022YFD1500502),安徽省水利水电勘测设计研究总院有限公司技术服务项目,现代农业产业技术体系专项资金(CARS-03-15, CARS-52)


Effects of Long-term Straw Burying and Nitrogen Fertilizer Application on Soil Bacterial Community Characteristics
Author:
Affiliation:

1.中国科学院南京土壤研究所;2.Fengqiu Argo-Ecological National Experimental Station;3.Henan University of Urban Construction, School of Surveying and Urban Spatial Information

Fund Project:

The National Key R&D Program of China(2022YFD1500903,2022YFD1500502)、Technical Service Project of Anhui Provincial Water Resources and Hydropower Survey, Design and Research Institute Co., Ltd and the Earmarked Fund for CARS(CARS-03-15, CARS-52)

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    为揭示典型潮土微生物群落及其生态网络对秸秆还田的响应机制,选择长期小麦-玉米轮作下不同秸秆还田处理的试验地为对象,应用高通量测序和生态网络等方法,阐明秸秆掩埋配施不同氮肥后土壤细菌群落组成、细菌网络共存关系以及与土壤养分的关联。结果表明:(1)与秸秆移除且不施氮肥处理相比,秸秆还田配合常规施肥处理显著降低土壤pH,提高了土壤全氮、有机碳、有效磷、速效钾和硝态氮含量(P < 0.05)。秸秆掩埋配施氮肥有利于增加土壤养分含量。(2)不同秸秆还田方式和氮肥施用量处理的细菌Alpha多样性无显著差异,而细菌群落结构差异显著。pH、SOC和TN等因子驱动了细菌群落结构变异。酸杆菌门、变形菌门、拟杆菌门和绿弯菌门是潮土细菌群落的主要优势菌门。(3)共现网络分析得到4个主要的生态集群,均与土壤养分有显著相关性。模块1中物种丰度与SOC、TN、TP、NO3- -N、AP和AK呈极显著负相关(P < 0.001),与pH呈极显著正相关(P < 0.001);模块2和模块3中物种丰度与大部分养分含量呈正相关,与pH呈负相关。因此秸秆还田配施氮肥可以通过调节细菌互作关系,从而调控土壤肥力。研究结果可为秸秆的资源化利用和土壤施肥管理提供科学依据。

    Abstract:

    【Objective】In order to reveal the response mechanism of microbial community and ecological network to straw returning process in typical fluvo-aquic soil, we experimented with different straw-returning treatments under long-term wheat-maize rotation. 【Method】The high-throughput sequencing and ecological network methods were utilized to analyze the soil bacterial community composition, bacterial network co-occurrence and their relationships with soil nutrient concentrations. 【Result】The results indicated that compared to straw removal and no nitrogen fertilizer treatment, straw returning with conventional fertilization treatments significantly reduced soil pH, while increasing the content of TN, SOC, AP, AK, NO3- -N (P < 0.05). The treatments of straw burying with nitrogen fertilizers were beneficial for increasing soil nutrient content. Moreover, no significant difference in bacterial alpha diversity was observed between different straw-returning methods and different amounts of nitrogen fertilizers, but a significant difference was observed in bacterial community structure. Factors such as pH, SOC, and TN drove variations in bacterial community structure.Also, Acidobacteriota, Proteobacteria, Bacteroidota and Chloroflexi were the dominant phyla in the fluvo-aquic soil. Furthermore, co-occurrence network analysis revealed four main ecological clusters that were significantly correlated with soil nutrients. The abundances of taxa in module 1 were found to be inversely correlated with SOC, TN, TP, NO3- -N, AP and AK (P < 0.001), and positively correlated with pH (P < 0.001). Conversely, the abundances of taxa in module 2 and module 3 were significantly positively correlated with most nutrient content, and negatively related to pH. 【Conclusion】Therefore, it can be concluded that straw burying combined with nitrogen fertilizers can improve soil nutrient by regulating microbial interactions. The findings of this study can provide a scientific basis for the efficient utilization of straw and the efficient management of soil fertilization.

    参考文献
    相似文献
    引证文献
引用本文

刘明凤,周桂香,张佳宝,张丛志,薛载琦,赵占辉.长期秸秆掩埋配施氮肥对土壤细菌群落特征的影响[J].土壤学报,DOI:10.11766/trxb202303090096,[待发表]
liumingfeng, zhouguixiang, zhangjiabao, zhangcongzhi, Xue Zaiqi, Zhao Zhanhui. Effects of Long-term Straw Burying and Nitrogen Fertilizer Application on Soil Bacterial Community Characteristics[J]. Acta Pedologica Sinica, DOI:10.11766/trxb202303090096,[In Press]

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2023-03-09
  • 最后修改日期:2023-05-30
  • 录用日期:2023-09-22
  • 在线发布日期: 2023-09-27
  • 出版日期: