[Objective] In recent years, the enrichment of soil-borne pathogens and antibiotic resistance genes (ARGs) has led to biological co-contamination of soil, posing a serious threat to agricultural product safety and human health. [Method] To investigate the synergistic remediation effect of reductive soil disinfestation (RSD) on soil biological co-contamination, intensive farmland soils (black soil, red soil and fluvo-aquic soil) with co-contamination of Ralstonia solanacearum, ARGs, and mobile genetic elements (MGEs) were selected. RSD was applied with 1% ethanol (ET,), alfalfa meal (AL, C/N: 21.2), and molasses (MO, C/N: 12.6), alongside controls of maximum water holding treatment (FCK) and untreated soil (CK). Real-time PCR was used to analyze the changes of R. solanacearum, major ARGs and MGEs before and after treatment, and the reduction rate of relative abundance was used to measure the mitigation effect of RSD treatment on soil biological co-contamination. [Result] The results showed that RSD could effectively reduce a variety of ARGs and MGEs, among which AL and MO treatment could decrease the relative abundance of aadA7, aadA21, tet36, sul1, and IS6100 genes in black soil, with a reduction rate of 28.4%~49.9%. After ET treatment, the relative abundance of aadA7, msrE, tetG, tetM, tet36, intl1, IS6100, and IS26 genes in fluvo-aquic soil decreased significantly, and the reduction rate reached 56.2%~81.6%. Additionally, RSD efficiently reduced the relative abundance of R. solanacearum in soil, and the decrease in red soil and fluvo-aquic soil was 88.0%~92.3% and 76.1%~94.2%, respectively. Correlation analysis showed that there was a certain coupling relationship between the relative abundance of R. solanacearum and ARGs and MGEs. In fluvo-aquic soil, the relative abundance of R. solanacearum was significantly and positively correlated with the relative abundances of most ARGs (aadA7, msrE, tetG, tetM, and tet36) and MGEs (intl1, IS6100, and IS26) genes, indicating that RSD treatment had a good synergistic reduction effect on the biological co-contamination in fluvo-aquic soil. Furthermore, there were considerable differences in the correlation results between the relative abundance of R. solanacearum, ARGs, and MGEs and soil physicochemical properties in different soil types. This indicates that the effectiveness of RSD treatment in remedying soil biological co-contamination varies depending on soil physicochemical properties. [Conclusion] RSD can synergistically reduce soil biological co-contamination caused by the superposition of soil R. solanacearum, ARGs, and MGEs, but its reduction effect is affected by soil and organic material types.