【Objective】 The soil organic carbon (SOC) pool is the largest carbon reservoir in terrestrial ecosystems, playing an essential role in mitigating climate change and maintaining soil fertility. Among the various components of SOC, microbial necromass carbon (MNC) constitutes a significant proportion, contributing approximately 30-80% to the total SOC, and playing a crucial role in stabilizing soil carbon stocks. Accumulation and stabilization of MNC in soil are closely linked to the formation and stability of soil aggregates, which provide physical protection against microbial decomposition. Despite the known connection between MNC and soil aggregation, no comprehensive studies have systematically explored the relationship between MNC and soil aggregate stability. This study aims to further explore the global association between MNC and soil aggregate stability.【Method】 To assess the relationship between MNC and soil aggregate stability, we compiled global observational datasets on soil amino sugars (biomarkers of MNC) and soil aggregates. Using machine learning techniques, we predicted the global distribution of MNC and analyzed its correlation with the stability of soil aggregates. The PLS-PM was employed to further investigate the pathways through which soil aggregate stability influences MNC sequestration, takin into account factors such as soil physical properties, nutrient availability. 【Result】 The results revealed that MWD is a key predictor of MNC, with a significant positive correlation between MNC and MWD on a global scale (P < 0.05). Further correlation analysis of global prediction data confirmed this relationship and showed that it is consistent across different ecosystems. The Partial Least Squares Path Model (PLS-PM) analysis revealed that soil aggregates protect MNC directly by forming physical barriers and indirectly by regulating soil physical properties and nutrient availability, which in turn influence MNC accumulation and stabilization. In particular, soil nutrients had the most significant positive impact on MNC (path coefficient = 0.67, P < 0.05). The process through which MWD influences MNC shows significant differences across different ecosystems, specifically in terms of the direction and strength of the pathways. For example, in agricultural ecosystems, the indirect effects through soil physical properties and nutrients are more pronounced, while in forest ecosystems, the direct effect is stronger. 【Conclusion】The findings of this study underscore the significant role of soil aggregates in stabilizing MNC, and highlight the potential of soil aggregation as a key factor in enhancing soil carbon storage. Also, the positive correlation between MNC and aggregate stability suggests that strategies aimed at improving soil structure; eg., practices that enhance aggregation and optimize nutrient management, can effectively contribute to greater carbon sequestration. By fostering more stable soil aggregates, we can improve MNC sequestration, mitigate climate change, and sustain soil fertility. Furthermore, these findings can inform the development of predictive models for MNC sequestration and the integration of soil aggregate stability as a critical indicator for assessing the carbon sequestration potential of soils.