城市绿地土壤细菌和真菌群落结构对有机物料的响应
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作者单位:

1.上海市园林科学规划研究院;2.上海市农业科学院生态环境保护研究所

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基金项目:

国家自然科学基金项目(面上项目,重点项目,重大项目)


Responses of Soil Bacterial and Fungal Community Characteristics to Organic Materials Application in Urban Green Soils
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Affiliation:

1.Shanghai Academy of Landscape Architecture Science and Planning;2.Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences

Fund Project:

The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)

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    摘要:

    添加有机物料显著影响土壤微生物群落结构及多样性,但城市绿地土壤微生物对有机物料的响应尚不明确。本研究采用尼龙网袋法,研究6种有机物料,包括园林绿化废弃物(绿废)、绿废堆肥、沼渣、沼渣堆肥、泥炭和生物炭,添加16个月后对城市绿地土壤理化性质、细菌和真菌群落特征及微生物网络关系的影响。结果表明,添加不同类型有机物料均提高城市绿地土壤电导率、有机碳和全氮含量,增幅分别为12.7%~49.0%、34.1%~87.0%和4.2%~14.7%。添加有机物料对细菌Alpha(?)多样性无影响,而显著促进真菌?多样性,土壤电导率是主要影响因子。子囊菌门(Ascomycota)是城市绿地土壤优势真菌,添加绿废和绿废堆肥处理土壤真菌群落显著区别于其他有机物料处理,pH和微生物生物量碳是影响真菌群落的重要因素;变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、绿弯菌门(Chloroflexi)和厚壁菌门(Fimicutes)等为土壤优势细菌,添加沼渣和沼渣堆肥与其他处理土壤细菌群落明显分离,有机物料芳香度指数是驱动细菌群落差异的主要因子。共现网络分析获得6个生态模块,不同模块中物种相对丰度与有机物料芳香度指数和土壤养分有较强相关性,其中,模块2、3、4中的细菌群落相对丰度在沼渣和沼渣堆肥处理中最高,其与土壤溶解性有机碳、微生物生物量碳和全氮含量呈显著正相关关系,表明添加沼渣和沼渣堆肥能提高土壤养分有效性,刺激细菌数量及活性。综上,不同类型有机物料可通过影响城市绿地土壤理化性质改变微生物群落组成和互作关系,进而调控土壤碳循环,添加低芳香度指数有机物料有利于碳的分解和周转,高芳香度指数有机物料则可能促进碳的稳定与留存。研究结果对于评价有机废弃物资源化利用协同提升城市绿地土壤微生物多样性及功能具有重要意义。

    Abstract:

    【Objective】Adding organic waste materials into soils significantly affects microbial characteristics, however, the responses of bacterial and fungal diversity, community compositions and their interactions to the addition of different types of organic materials in urban green soils remain poorly understood.【Method】Using mesh bag method, six types of organic materials including green waste (GW), green waste compost (GWC), biogas residue (BR), biogas residue compost (BRC), peat (PT) and biochar (BC) were selected to investigate the effects of organic materials addition on soil properties, microbial communities and co-occurrence network in urban green soils through a 16-month in situ experiment.【Result】The addition of organic materials greatly increased soil electrical conductivity, soil organic carbon, and soil total nitrogen content by 12.7%-49.0%, 34.1%-87.0%, and 4.2%-14.7%, respectively. Soil bacterial alpha (?) diversity did not change among all the treatments, while soil fungal ? diversity was obviously increased after organic materials addition, which was mainly regulated by soil electrical conductivity. The dominant fungi were Ascomycota in urban green soils. Fungal communities in GW and GWC treatments obviously differed from other treatments, which was significantly influenced by soil pH and microbial biomass carbon. In contrast, Proteobacteria, Acidobacteria, Chloroflexi and Fimicutes were abundant in urban green soils. Bacterial communities in BR and BRC treatments were distinctly separated from other treatments, which was primarily driven by the aromaticity index of organic materials. Further analysis of occurrence-network revealed six main ecological clusters. The relative abundances of microbe in each module were different among all the treatments and were significantly correlated with soil nutrients and aromaticity index of organic material. Specifically, the highest relative abundance of bacteria community in module 2, 3 and 4 was observed in BR and BRC treatments, which was positively correlated with dissolved organic carbon, microbial biomass carbon, and soil total nitrogen, indicating that addition of biogas residue and biogas residue compost might enhance soil nutrient availability and subsequently facilitate microbial activity.【Conclusion】This study concludes that adding different types of organic materials can regulate urban green soil microbial community composition and interaction patterns by influencing soil physicochemical properties, thereby altering soil carbon cycling. Organic materials with low aromaticity index, which are more easily decomposed by microorganisms, may accelerate soil carbon cycling, whereas organic materials with high aromaticity index may favor carbon retention in soils. These findings hold significant implications for accurately assessing the resource utilization of urban organic wastes and the improvement of microbial diversity and ecological function in green space soils.

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牛玉慧,王庆峰,马想,何小丽,梁晶.城市绿地土壤细菌和真菌群落结构对有机物料的响应[J].土壤学报,DOI:10.11766/trxb202412230502,[待发表]
Niu Yuhui, Wang Qingfeng, Ma Xiang, He Xiaoli, Liang Jing. Responses of Soil Bacterial and Fungal Community Characteristics to Organic Materials Application in Urban Green Soils[J]. Acta Pedologica Sinica, DOI:10.11766/trxb202412230502,[In Press]

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  • 收稿日期:2024-12-23
  • 最后修改日期:2025-07-15
  • 录用日期:2025-09-04
  • 在线发布日期: 2025-09-05
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