减施化肥配施有机物料对旱地红壤团聚体尺度上线虫群落的影响
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1.南京农业大学资源与环境科学学院土壤生态实验室;2.兰州大学草地微生物中心

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国家自然科学基金项目(42477322)资助


Effect of Organic Amendments on Nematode Community Under Reduced Chemical Fertilizer Application at the Aggregate Scale in Red Soil of Dryland
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1.Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University;2.Centre for Grassland Microbiome, State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University

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Supported by the National Natural Science Foundation of China (No. 42477322)

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    摘要:

    为充分满足不断增长的人口对农产品的需求,减少化肥施用、投入有机物料、提高养分利用效率已被认为是保障粮食安全的有效途径。不同粒径的土壤团聚体在水热条件与养分可利用性方面存在显著差异,因而对线虫群落的调控作用也有所不同。然而,在团聚体尺度上,减施化肥并配施有机物料对土壤线虫的影响仍知之甚少。因此,在旱地红壤农田基于化肥施用量(设置两个水平:全量化肥氮磷钾( NPK )和减量 60%的 NPK)与有机物料类型(不施加有机物料、施加秸秆、施加生物质炭)之间的相互作用,总共设计了6个处理,每个处理将土壤筛分为4种团聚体尺度(> 2 mm, 2~1 mm, 1~0.25 mm, < 0.25 mm)。结果表明:减施化肥下所有处理的线虫数量均高于全量化肥处理;无论化肥减施与否,施加秸秆和生物质炭均导致了线虫总数的上升,并且线虫数量随着土壤团聚体粒径的增加而增加。与施用秸秆相比,施用生物质炭总体上减少了食微线虫的数量但增加了捕食杂食线虫的数量。此外,于2~1 mm粒径团聚体这一尺度上,相较于秸秆,添加生物质炭降低了食细菌线虫和捕食杂食线虫,但增加了食真菌线虫数量。通过线虫区系分析发现,随着团聚体粒径的减小,土壤线虫的结构指数与富集指数均呈下降趋势,表明线虫群落受外界环境干扰较大;进一步分析表明,在大团聚体(>1 mm)尺度上土壤含水量是有机物料影响土壤线虫群落结构的关键因素,而在微团聚体(<0.25 mm)尺度上,土壤有机碳是关键因子(P <0.01)。综上所述,有机物料、化肥减施和团聚体粒径及其交互作用均显著影响线虫群落。在农业生产中,应合理选用有机物料类型,与化肥减施相结合,以更好地保护土壤健康,指导农业生产。

    Abstract:

    【Objective】In order to adequately meet the growing population"s demand for agricultural products, reducing the application of chemical fertilizers, investing in organic amendments, and improving the efficiency of nutrient use have been recognized as effective ways to guarantee food security. Different soil aggregate sizes exhibit significant variations in terms of water-heat conditions and nutrient distribution, which in turn influence nematode communities to varying extents. However, the impact of reduced chemical fertilizer application combined with different organic amendments on soil nematodes at the aggregate scale remain poorly understood. 【Method】This study investigated the interaction between reduced fertilizer application (2 levels: full chemical fertilizer NPK and 60% reduction in NPK) and organic amendment types (no organic amendment as control, straw, and biochar) in the red soil of dryland agriculture. Each treatment was further divided into four aggregate size classes (> 2 mm, 2-1 mm, 1-0.25 mm, < 0.25 mm). 【Result】The results indicated that the nematode abundance in all treatments with reduced fertilizer application was higher than that of the full chemical fertilizer treatment. Regardless of the fertilizer application, both straw and biochar significantly increased the total nematode abundance, and nematode numbers increased with the increase in aggregate size. Compared to straw application, biochar application generally decreased the abundance of bacterivorous nematodes while increasing the abundance of omnivorous nematodes. Notably, in the 2-1 mm aggregate size class, biochar addition decreased the abundance of bacterivorous and omnivorous nematodes compared to straw, but increased the abundance of fungivores nematodes. Nematode community analysis revealed that as aggregate size decreased, the nematode structure and enrichment indices tended to decrease, indicating greater environmental disturbance. Further analysis showed that, soil pH and moisture were key factors influencing nematode community structure at the larger aggregate scale, while soil organic carbon was the critical factor at the smaller aggregate scale (P<0.01). 【Conclusion】This study revealed that organic amendments, reduced fertilizer application, aggregate size, and their interactions significantly affected nematode communities. Therefore, the rational combination of organic amendment types and reduced fertilizer application should be considered to better protect soil health and guide agricultural production.

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杨佳妮,朱柏菁,任竹红,万兵兵,陈小云,刘满强.减施化肥配施有机物料对旱地红壤团聚体尺度上线虫群落的影响[J].土壤学报,2026,63(1). DOI:10.11766/trxb202501070013 YANG Jiani, ZHU Baijing, REN Zhuhong, WAN Bingbing, CHEN Xiaoyun, LIU Manqiang. Effect of Organic Amendments on Nematode Community Under Reduced Chemical Fertilizer Application at the Aggregate Scale in Red Soil of Dryland[J]. Acta Pedologica Sinica,2026,63(1).

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  • 收稿日期:2025-01-07
  • 最后修改日期:2025-06-26
  • 录用日期:2025-07-29
  • 在线发布日期: 2025-07-30
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