耐盐藻菌的筛选及其联合体在盐渍化土壤改良中的作用研究
CSTR:
作者:
作者单位:

西南大学

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金项目(42477057)和大学生创新创业训练计划项目(X202410635664)资助


Screening of Salt-tolerant Algae and Bacteria and the Role of Theirs Consortium in Salinized Soil Improvement
Author:
Affiliation:

Southwest University

Fund Project:

Supported by the National Natural Science Foundation of China (No.42477057), and the College Students’ Innovative Entrepreneurial Training Plan Program (No.X202410635664)

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    土壤盐渍化不仅导致土壤的理化性质恶化,而且严重危害作物的生长发育,对农业生产和生态环境造成严重危害。为探究盐渍化土壤改良的新型生物技术,本研究从盐渍化土壤中分离了5株微藻和10株细菌,通过耐盐初筛及藻菌联合能力复筛,筛选出Borodinellopsis sp.和芽孢杆菌(Bacillus sp.)最优组合,并将其应用于盐渍化土壤改良中,分析其对土壤理化性质的影响。结果表明,经过10 d的培养,在15 g·L-1和20 g·L-1盐胁迫下,藻菌组合胞外聚合物(EPS)分泌量分别显著增加了44.56%和43.19%;溶液可溶性盐含量均显著下降,去盐率分别达57.89%和57.55%。将Borodinellopsis、芽孢杆菌和藻菌组合分别接种至盐渍化土壤表层培养30 d,土壤EPS含量均显著增加,分别增加了51.72%、8.20%和185.88%;土壤浸出液盐度较空白均显著下降,分别降低了5.10%、3.45%和7.00%,其中接种藻菌组合的土壤浸出液盐度显著低于单独接种藻或菌的土壤;同时,藻菌联合处理组土壤中的全氮含量较空白显著提高了55.33%,而接种单一藻或菌的土壤总氮含量无明显变化。综上,土壤微藻和细菌具有降低土壤盐分的作用,且藻菌联合对盐渍化土壤的改良效果优于单独应用藻或菌的效果。本研究旨在为藻菌联合改良盐渍化土壤提供重要的理论依据。

    Abstract:

    【Objective】Soil salinization can not only lead to the deterioration of soil physical and chemical properties, but also can seriously harm the growth and development of crops, causing severe harm to agricultural production and the ecological environment. Using microorganisms to improve the quality of salinized soil is a clean and efficient way, but there are also problems, such as the single function of microorganisms and the limited improvement effect. Nevertheless, and algae-bacterial consortium can fully exert the synergistic effect of algae and bacteria and enhance the effect of algae and bacteria on soil. However, at present, there are few related studies on the improvement of salinized soil using the algae-bacterial consortium.【Method】In this study, five microalgae and ten bacteria were isolated from salinized soil. After comparing the changes of biomass and specific growth rate in algae or bacteria under different salt stress, three salt-tolerant algae and four salt-tolerant bacteria were screened out. Afterwards, the three salt-tolerant algae and four salt-tolerant bacteria were combined into different algae-bacterial consortia. By comparing the changes in photosynthetic pigment content of algae in each algae-bacterial consortium, three salt-tolerant algae-bacterial consortia were further selected. Subsequently, the changes in EPS secretion and desalination effects were analyzed under different degrees of salt stress, and the optimal consortium of algae-bacteria was identified. Moreover, 18S-rDNA sequencing or 16S-rDNA sequencing was performed on algae-1 and bacteria-8, and the phylogenetic trees were constructed using the neighbor-joining method, respectively. Finally, the algae1-bacteria8 consortium was applied to salinized soils to improve soil quality.【Result】According to the constructed phylogenetic tree, algae-1 was identified as Borodinellopsis sp., and bacteria-8 belonged to Bacillus sp. After 10-day culture, the exopolysaccharide (EPS) secretion of algae and bacteria increased significantly by 44.56% and 43.19% at treatments of 15 g·L-1 and 20 g·L-1 salt stress, respectively. The soluble salt content of the solution decreased significantly, and the salt removal rate reached 57.89% and 57.55% at treatments of 15 g·L-1 and 20 g·L-1, respectively. When Borodinellopsis, Bacillus, and algae-bacterial consortium were respectively inoculated into salinized soil surface for 30 days, EPS content in soil was significantly increased by 51.72%, 8.20% and 185.88%, and the salinity of soil leaching solution decreased by 5.10%, 3.45% and 7.00%, respectively. Moreover, the salinity of the soil leaching solution inoculated with an algae-bacterial consortium was significantly lower than that of the soil inoculated with algae or bacteria alone. Concurrently, the total nitrogen content of soil in algae-bacterial consortium was significantly increased by 55.33% compared with the initial group, while no significant changes were observed in treatments of algae or bacteria alone.【Conclusion】Collectively, although both soil microalgae and bacteria can reduce soil salinity, algae-bacterial consortium have better effects on salinized soil than algae or bacteria alone. This study will provide an important theoretical basis for improving salinized soil with an algae-bacterial consortium.

    参考文献
    相似文献
    引证文献
引用本文

舒 雪,赵晨宇,施军琼,吴忠兴.耐盐藻菌的筛选及其联合体在盐渍化土壤改良中的作用研究[J].土壤学报,2026,63(1). DOI:10.11766/trxb202501240041 SHU Xue, ZHAO Chenyu, SHI Junqiong, WU Zhongxing. Screening of Salt-tolerant Algae and Bacteria and the Role of Theirs Consortium in Salinized Soil Improvement[J]. Acta Pedologica Sinica,2026,63(1).

复制
分享
相关视频

文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2025-01-24
  • 最后修改日期:2025-06-30
  • 录用日期:2025-07-28
  • 在线发布日期: 2025-07-31
  • 出版日期:
文章二维码