【Objective】Clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/Cas)-mediated gene editing technology has been widely applied in molecular plant breeding. However, the effects of genotypic variation induced by this technology on the structure and potential functions of soil bacterial communities constitute a central issue in environmental safety assessment.【Method】The herbicide-resistant gene-edited rice SF24HI708, carrying mutations in the Acetyl-CoA carboxylase (ACCase) gene and its parental cultivar Huanghuazhan (HHZ) were used as model plant materials. High-throughput sequencing of 16S rRNA genes was employed to characterize the structure and functional metabolic pathways of rhizosphere bacterial community at the tillering and heading stages.【Result】Neither genotype nor growth stage significantly affected the α-diversity indices of rhizosphere bacterial communities, including the Abundance-based Coverage Estimator (ACE), Chao 1, Shannon and Simpson indices. However, both factors significantly altered community composition. In both rice lines, the assembly patterns of rhizosphere bacterial communities at the tillering and heading stages was predominantly governed by stochastic processes, with drift and other processes each contributing ≥68%. Co-occurrence network analysis further revealed that the rhizosphere bacterial community associated with the gene-edited rice exhibited higher stability than that of the parental cultivar. Kyoto Encyclopedia of Genes and Genomes(KEGG) functional analysis showed that, compared with the parental cultivar, the sphingolipid signaling pathway was significantly upregulated in the gene-edited rice at the tillering stage. At the heading stage, metabolic pathways involved in the synthesis and degradation of lipids and polysaccharides were significantly downregulated, whereas key pathways related to energy metabolism and substance transport were significantly upregulated.【Conclusion】Although gene-edited rice changed the composition and potential functional pathways of the rhizosphere bacterial community, it did not lead to decreased alpha diversity indexs or weakened community stability, indicating that its cultivation had no adverse effects on rhizosphere microecology.