【Objective】 This study aimed to investigate the occurrence characteristics, bioaccumulation and translocation behaviors, and dietary risks of per- and polyfluoroalkyl substances (PFASs) in rice and surrounding farmland near a fluorochemical industrial park in Changshu, Jiangsu Province, China. 【Method】 Rice plants, rhizosphere soil, and irrigation water samples were collected from five paddy fields surrounding the industrial park. A total of 37 PFASs were quantified by weak anion exchange solid-phase extraction coupled with liquid chromatography–tandem mass spectrometry. Bioaccumulation factors (BAF) and translocation factors (TF) were applied to characterize the accumulation and translocation behaviors of PFASs within rice tissues. Dietary risks were further assessed based on PFAS concentrations in brown rice and white rice. 【Result】 PFASs were ubiquitously detected in rice, soil, and irrigation water, with 28, 22, and 24 PFASs identified, respectively. PFASs in rice showed a distinct organ-specific distribution pattern, following the order: leaf > root > stem > brown rice > white rice. The predominant pollutants included PFBA, PFOA, and the emerging alternatives GenX and F-53B. The emerging alternatives showed relatively high translocation and accumulation potential in rice grains, whereas short-chain PFCAs and PFOA were more likely to migrate to and accumulate in vegetative tissues. Health risks varied among residential groups and age groups, with rural residents showing higher overall risks than urban residents and children aged 2–6 years identified as the most sensitive population. Brown rice posed a higher dietary exposure risk than white rice. Hazard quotient results indicated that dietary risks were jointly driven by emerging alternatives (GenX and F-53B) and traditional PFASs (PFOA and PFOS). 【Conclusion】 Fluorochemical industrial activities have exerted clear impacts on the surrounding agricultural ecosystem. The exposure risks associated with emerging alternative PFASs should not be neglected, while the potential health risks posed by traditional PFASs such as PFOA and PFOS through rice consumption also warrant continued attention.