煤矸石山复垦表土建植受限因素及细菌群落结构响应
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1.中国矿业大学矿业工程学院;2.中国矿业大学环境与测绘学院;3.中国科学院南京土壤研究所;4.中国科学院生态环境研究中心

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国家重点研发计划项目(2023YFC3804203)和国家自然科学基金项目(52504193, 52474198)资助


Limiting Factors for Revegetation and Bacterial Community Structure Responses in Reclaimed Topsoil of Coal Gangue Dumps
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Affiliation:

1.School of Mines, China University of Mining and Technology, Xuzhou;2.School of Environment and Spatial Informatics, China University of Mining and Technology, Xuzhou;3.Institute of Soil Science, Chinese Academy of Sciences;4.Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences

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Supported by the National Key Research and Development Program of China (No. 2023YFC3804203) and the National Natural Science Foundation of China (Nos. 52504193 and 52474198)

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    摘要:

    煤矸石山复垦通常采用表土覆盖与人工建植的方式,但复垦后部分区域仍难以形成稳定植被,识别其受限因素是科学指导煤矸石山生态修复工程的重要基础。以山西阳泉四座分别在1990、2005、2012和2018年完成复垦的煤矸石山为对象,对同一矸石山内部已建植样地与建植受限样地配对采样,探究表土理化性质、结构稳定性、酶活性、细菌丰度与群落组成差异,并结合响应比、分组曼特尔(Mantel)检验和偏最小二乘路径模型(PLS-PM)解析建植受限的表征因素及潜在作用路径。结果表明:硫组分累积与水分、养分不足是建植受限表土最突出的特征,建植受限样地在四个复垦年份中总硫分别为2.40、3.63、5.08和7.65 gkg-1,硫酸盐硫分别为337.2、528.8、863.7和1 947 mgkg-1;已建植样地容重在四个复垦年份中均显著低于建植受限样地,结构稳定性高;酶活性和细菌α多样性响应方向不稳定,而16S rRNA基因拷贝数在四个复垦年份中均显著高于建植受限样地,表明细菌丰度对建植状态分化具有稳定响应;各样地均以Micrococcaceae为优势类群,主坐标分析(PCoA)和置换多元方差分析(PERMANOVA)表明细菌群落结构分异显著;细菌群落Bray-Curtis距离与硫盐约束相关性最强;硫盐约束对水分、养分、结构稳定性和细菌响应均为显著负效应,PLS-PM揭示了表土限制因子间的相关关系。综上,硫盐累积、结构压实、水分和养分不足及细菌丰度降低共同构成建植受限表土障碍因素,总硫、硫酸盐硫、容重和16S rRNA基因拷贝数可作为优先诊断指标。研究结果可为煤矸石山表土质量诊断与生态修复调控提供参考。

    Abstract:

    【Objective】Coal gangue dumps are commonly reclaimed through topsoil covering and artificial planting, yet stable vegetation remains difficult to establish in some reclaimed areas. Identifying the associated constraints is essential for facilitating ecological restoration. 【Method】Four coal gangue dumps reclaimed in 1990, 2005, 2012, and 2018 in Yangquan, Shanxi Province, were investigated. Well-vegetated and revegetation-limited sites within each dump were sampled in pairs. Topsoil physicochemical properties, structural stability, enzyme activities, bacterial abundance, and community composition were analyzed. Response ratios, grouped Mantel tests, and partial least squares path modeling (PLS-PM) were used to identify stable indicators and potential pathways of vegetation limitation. 【Result】Sulfur accumulation and insufficient water and nutrient supply were the most prominent characteristics of vegetation-limited topsoil. In the revegetation-limited sites of the four reclamation years, total sulfur contents were 2.40, 3.63, 5.08, and 7.65 g?kg?1, and sulfate sulfur contents were 337.2, 528.8, 863.7, and 1 947 mg?kg?1, respectively. Bulk density in well-vegetated sites was significantly lower than that in revegetation-limited sites across all four reclamation years, indicating higher structural stability. Enzyme activities and bacterial alpha diversity showed unstable response directions, whereas 16S rRNA gene copy numbers were significantly higher in well-vegetated sites than in revegetation-limited sites across all four reclamation years, indicating that bacterial abundance showed a stable response to revegetation-status differentiation. Micrococcaceae was the dominant taxon in all sites. Principal coordinates analysis (PCoA) and permutational multivariate analysis of variance (PERMANOVA) showed significant differentiation in bacterial community structure. Bacterial Bray-Curtis dissimilarity was most strongly correlated with sulfur-salinity constraints. Sulfur-salinity constraints had significant negative effects on water supply, nutrient supply, structural stability, and bacterial response. The PLS-PM further revealed correlations among topsoil limiting factors. 【Conclusion】Sulfur-salinity accumulation, structural compaction, insufficient water and nutrient supply, and reduced bacterial abundance jointly formed the topsoil barrier to revegetation establishment. Total sulfur, sulfate sulfur, bulk density, and 16S rRNA gene copy number can serve as priority diagnostic indicators. These findings provide a scientific basis for topsoil quality diagnosis and ecological restoration of coal gangue dumps.

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计楚柠,卞正富,胡振琪,沈仁芳,陈保冬.煤矸石山复垦表土建植受限因素及细菌群落结构响应[J].土壤学报,DOI:10.11766/trxb202604210201,[待发表]
JI Chuning, BIAN Zhengfu, HU Zhenqi, SHEN Renfang, CHEN Baodong. Limiting Factors for Revegetation and Bacterial Community Structure Responses in Reclaimed Topsoil of Coal Gangue Dumps[J]. Acta Pedologica Sinica, DOI:10.11766/trxb202604210201,[In Press]

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  • 收稿日期:2026-04-21
  • 最后修改日期:2026-07-21
  • 录用日期:2026-09-02
  • 在线发布日期: 2026-09-04
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