土壤中具有蛋白酶活性的酶-腐殖质复合物的提取及其性质的研究
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EXTRACTION AND PROPERTIES OF ENZYME-HUMUS COMPLEXES WITH PROTEASE ACTIVITY IN SOIL
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    摘要:

    用中性0.1M焦磷酸钠提取,经硫酸铵分段,丙酮沉淀,从土壤中获得了具有蛋白酶活性的酶-腐殖质复合物,其特性:反应最适pH为9.0,反映最适温度为50°-60℃,温度系数Q10(17°-40℃)为1.93,Km值为0.97-1.00毫克酪素/毫升,Vmax值为74.91-75.35毫克酪氨酸/克·24小时,与原位土壤蛋白酶相比,反应最适pH往碱性偏移,反应最适温度及Km值变小,热稳性降低,Q10和Vamx值增大.土壤经超声波分散后,可以用各种盐缓冲液将其中具有蛋白酶活性的酶-腐殖质复合物提取出来,其收率依次为水<0.1M磷酸盐-0.3M KCl-0.01MEDTA<三羟甲基氨基甲烷(pH7)<0.2M碳酸氢钠<0.05M磷酸盐-柠檬酸<0.1M焦磷酸钠溶液.

    Abstract:

    The extraction of the enzyme-humus complexes with protease activity from soil using a series buffered eatractants was studied. It was shown that the extraction of 0.1 M pyrophosphate (pH 7.0) was most efficient. Some characteristics of the extraeted enzyme were different from those of the enzyme measured in soil. The reasults also showed than the Km value (0.97-1.0mg casein/ml) and optimum temperature (50°-60℃) for the extracted enzyme were lower than those of the enzyme in soil and the Vmax value (74.91-75.35 mg tyrosine/g · 24hr.) optimum pH (9) and temperature coefficient (1.93) were higher than those of the enzyme in soil. The effectiveness of the estractants was in order of distilled water<0.1 M phosphate (pH 7.0)-0.3 M KCl-0.01 M EDTA 3 <0.05 M phosphate-citrate (pH 7.0) <0.1 M Na-Pyrosphate (pH 7.0).

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郑洪元,张德生,郑莲娣.土壤中具有蛋白酶活性的酶-腐殖质复合物的提取及其性质的研究[J].土壤学报,1985,22(4):357-364. Zheng Hongyuan, Zhang Desheng, Zheng Liandi. EXTRACTION AND PROPERTIES OF ENZYME-HUMUS COMPLEXES WITH PROTEASE ACTIVITY IN SOIL[J]. Acta Pedologica Sinica,1985,22(4):357-364.

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  • 在线发布日期: 2013-02-25
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