This paper describes the structure and function of plant sterile culture laboratory in details. Some key steps in plant sterile culture such as sterilization of nutrient solution and culture of aseptic seedlings were tested successfully. Results from sterile experiment for rice organic N nutrition showed that amino acid could be directly taken up by plants. The effectiveness of N sources at equal N concentration (N 10mg/L) for rice followed the order: glycine-N (sterile culture)>glutamic acid-N (sterile culture)>ammonium sulphate-N (sterile culture)>ammonium sulphate-N (conventional culture) under frequent renewal of nutrient solution in the experiment. This indicated clearly the differences among the effciency of various organic and inorganic N compounds, and sterile and conventional growth conditions on rice N nutrition.