两株发光酶基因标记的重组大豆根瘤菌在土壤缩影中的存活研究
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*国家863高技术研究发展计划课题(2001AA214021);国家973重点基础研究发展规划项目(001CB1089)资助


STUDY ON POPULATION SURVIVAL OF TWO RECOMBINANT SOYBEAN RHIZOBIA MARKED WITH luxAB GENE IN SOIL MICROCOSM
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    摘要:

    研究了发光酶基因(luxAB)标记的重组费氏中华根瘤菌HN01DL、大豆慢生根瘤菌TA113QD与其出发菌株HN01、TA11在灭菌和未灭菌土缩影中的存活动态.结果表明:HN01DL和TA113QD在灭菌土缩影和未灭菌土缩影中的存活动态特征显著不同:标记菌株在灭菌土缩影中的早期存活数量一般有所下降后而保持相对稳定的较高存活水平,而在未灭菌土缩影中其存活数量在整个跟踪过程中持续下降至约45logcfug-1土.在本实验中未发现供试标记重组菌株和出发菌株在土壤缩影中的存活性存在显著差异.

    Abstract:

    The survival dynamics of recombinant Sinorhizobium fredii HN01DL,Bradyrhizobium japonicum TA113QD both of which marked with luxAB gene and its wild-type strain HN01,TA11 in sterilized and non-sterilized soil microcosm were studied respectively1 The results showed that the population dynamics of HN01Dl and TA113QD in sterilized and non-sterilized soil microcosm was obviously different1 The survival population of the marked rhizobia decreased slightly at an early stage after inoculation and then kept a relatively stable high survival level in sterilized soil microcosm,and its survival population in non-sterilized soil microcosm decreased regularly at a high speed until reaching about 4.5 log cfu g-1 soil1 No significant difference in soil survival was found between the recombinant marked strains and the wild-type stains.

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李友国,周俊初.两株发光酶基因标记的重组大豆根瘤菌在土壤缩影中的存活研究[J].土壤学报,2003,40(4):613-617. DOI:10.11766/trxb200111140420 Li You-guo, Zhou Jun-chu. STUDY ON POPULATION SURVIVAL OF TWO RECOMBINANT SOYBEAN RHIZOBIA MARKED WITH luxAB GENE IN SOIL MICROCOSM[J]. Acta Pedologica Sinica,2003,40(4):613-617.

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  • 收稿日期:2001-11-14
  • 最后修改日期:2002-07-26
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  • 在线发布日期: 2013-02-25
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