en known strains of bacteria were used to study validity of the Sherlock Microbial Identification System(Sherlock MIS) in identification of soil bacteria.Results show that Sherlock MIS is accurate in identifying all the bacteria,incubated in TSBA(Trypticase soy broth agar),except Bacillus thuringiensis,whereas it is not so ideal,when the bacteria are incubated in BPA(beef peptone agar).A better result can be obtained when the conserved bacteria are activated twice or for 48 hours than for 24 hours.No difference is found in identification between bacteria sampled from quadrant 3 and quadrant 2.