一株具有多途径氮代谢功能的荧光假单胞菌
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* 江苏省自然科学基金项目(BK2002019)资助


A STRAIN OF PSEUDOMONAS FLUORESCENS WITH MULTI-PATHS OF NITROGEN METABOLISM
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    摘要:

    为研究全自养脱氮过程,采用斯凯尔曼亚硝化单胞杆菌培养基,从南京某城市污水处理厂序批式处理系统(SBR)中分离得到一株具有多途径氮代谢功能的荧光假单胞菌Pseudomonas fluorescens dN13。该菌株在有氧条件下,无有机碳源存在,能进行氧化NH3-N产能代谢生长;有有机碳源存在且NO2--N和NO3--N的浓度较高时,它能将NO2--N和NO3--N还原为NH3-N。在厌氧条件下,只有乙酸钠作碳源时,该菌株仍能进行硝酸亚硝酸还原(反硝化)作用。在这个具有多途径氮代谢功能的菌株细胞内,硝化反硝化过程耦合成为可能。

    Abstract:

    To study on autotrophic removal of nitrogen from wastewater containing a high concentration of NH3-N,a strain of Pseudomonas fluorescens dN13 was isolated,by means of incubation in Skerman's medium for Nitrosomona,from the sequence batch reactor system(SBR)of a wastewater treatment plant in Nanjing.Under aerobic condition,this strain could oxidize NH3-N into NO2--N and grow without organic carbon source;and reduce NO2--N and NO3--N into NH3-N with organic carbon source and adequate concentration of NO2--N and NO3--N,while under anaerobic condition,strain dN13 could still reduce NO2--N and NO3--N into NH3-N with sodium acetate as the sole carbon source.In the cells of strain dN13 with such multi-paths of nitrogen metabolism,coupling of nitrification and denitrification is feasible.

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王晋宇,范伟平,李霜,沈珈琦.一株具有多途径氮代谢功能的荧光假单胞菌[J].土壤学报,2007,44(1):144-149. DOI:10.11766/trxb20070121 Wang Jinyu, Fan Weiping, Li Shuang, Shen Jiaqi. A STRAIN OF PSEUDOMONAS FLUORESCENS WITH MULTI-PATHS OF NITROGEN METABOLISM[J]. Acta Pedologica Sinica,2007,44(1):144-149.

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  • 收稿日期:2005-10-27
  • 最后修改日期:2006-01-08
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  • 在线发布日期: 2013-02-25
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