Although the enhanced biological phosphorus removal (EBPR) process is widely applied in wastewater treatment to remove phosphorus biologically, it is difficult to predict and control its effect, which is unstable and would often decline suddenly in large-scale municipal sewage treatment plants, on account of lack of knowledge about microbiological and molecular mechanisms of the EBPR process.An experiment was carried out using Pseudomonas putida GM6, a strain of high-efficiency phosphorus-accumulating bacteria isolated and identified in the lab, to quickly reactivate the phosphorus removing capacity of sludge.In order to learn how GM6 colonize in the reactor, Pseudomonas putida GM6 was first labeled with gfp gene through triparent conjugation, and then introduced into a sequencing batch reactor (SBR) installation, low in phosphorus removing efficiency in the lab to investigate how GMTR colonize in activated sludge.The initial count of GMTR introduced into the sludge accounted for 1.5%～3% of the total count of bacteria in the sludge, and rose up to 9.2% after 21 days, indicating GMTR colonized firmly in the sludge.At the same time, variation of phosphorus-accumulating capacity of R2 was studied after inoculation.It was found that phosphorus removal rate gradually increased 5 days after inoculation, and up to 96% at D 21.And phosphate concentration in the effluent lingered around 0.2 mg L^-1 after 28 days.The findings demonstrate that the strain of Pseudomonas putida GM6 is quick to initiate its biological phopshorus removing function in wastewater and may serve as scientific basis for large-scale engineering application.