引用本文:聂军,周健民,王火焰,陈小琴,杜昌文.长期施肥条件下不同粒径土壤团聚体中Bradford反应土壤蛋白质含量的差异[J].土壤学报,2008,45(6):1072-1080. DOI:trxb10.11766/200701210609
Nie Jun,Zhou Jianmin,Wang Huoyan,Chen Xiaoqin,Du Changwen.BRADFORD REACTIVE SOIL PROTEIN CONCENTRATIONS IN AGGREGATES DIFFERENT IN PARTICLE SIZE UNDER LONG-TERM FERTILIZATION[J].Acta Pedologica Sinica,2008,45(6):1072-1080. DOI:trxb10.11766/200701210609
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长期施肥条件下不同粒径土壤团聚体中Bradford反应土壤蛋白质含量的差异
聂军1,2,3,4, 周健民1, 王火焰1, 陈小琴1, 杜昌文1
1.中国科学院南京土壤研究所, 南京 210008;2.湖南省土壤肥料研究所, 长沙 410125;3.中国科学院研究生院, 北京 100049;4.农业部望城红壤水稻土生态环境重点野外科学观测试验站, 长沙 410125
摘要:
主要研究了稻—稻连作方式下长期不同施肥对不同粒径土壤团聚体中Bradford反应土壤蛋白质(BRSP)含量的影响及BRSP与土壤有机碳(SOC)和全氮(TN)之间的关系,同时为简化BRSP测定过程,探讨了用过1mm筛土壤代替1~2mm土壤团聚体研究BRSP含量的可行性.研究结果表明,长期不同施肥显著影响土壤1~2mm团聚体中总BRSP(T-BRSP)和易浸提BRSP(EE-BRSP)含量.有机无机肥料配合施用能显著提高土壤中BRSP含量,以NPK+稻草还田提高BRSP含量的效果最佳.经25a施用不同肥料后,1~2mm土壤团聚体中2种形态BRSP含量与土壤SOC和TN含量呈显著正相关.试验发现,<1mm土壤团聚体中同样有BRSP,且处理间的变化趋势与1~2mm团聚体间的变化趋势相似.试验结果显示,过1mm筛土壤中T-BRSP和EE-BRSP含量与1~2mm团聚体中含量的相关性均达到极显著水平,且土壤SOC和TN含量的相关性也均达到极显著水平.
关键词:  长期施肥  不同粒径团聚体  Bradford反应土壤蛋白质  土壤
基金项目:国家重点基础研究发展规划(973)项目(2002CB410810)资助
BRADFORD REACTIVE SOIL PROTEIN CONCENTRATIONS IN AGGREGATES DIFFERENT IN PARTICLE SIZE UNDER LONG-TERM FERTILIZATION
Nie Jun1,2,3,4, Zhou Jianmin1, Wang Huoyan1, Chen Xiaoqin1, Du Changwen1
1.Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;2.Soil and Fertilizer Institute of Hunan, Changsha 410125, China;3.Graduate University of Chinese Academy of Sciences, Beijing 100049, China;4.Key Field Monitoring Experimental Station for Reddish Paddy Soil Eco-environment in Wangcheng, Ministry of Agriculture of China, Changsha 410125, China
Abstract:
Studies were carried out on B radford reactive soil protein(BRSP) concentrations in aggregates different in particle size and relations of BRSP with soil organic C and total N in fields of long-term fertilization experiment under a continuous rice-rice cropp ing system.At the same time,in order to simplify the process of the soil BRSP test,attempts were made to exp lore feasibility of determining soil BRSP concentration using whole soil passed through 1 mm sieve instead of 1~2 mm soil aggregates.Results show that long-term fertilization significantly affected concentrations of soil total BRSP(T-BRSP) and easily extractable BRSP(EE-BRSP) in 1~2 mm aggregate.Soil BRSP was increased sharply by application of inorganic fertilizer p lus organic manure,especially in the treatment of N,P,K p lus rice straw.After 25 years of continuous fertilization,regardless of treatments,both T-BRSP and EE-BRSP in 1~2 mm aggregates were highly correlated with SOC and TN.It was found in the experiment that BRSP existed in < 1 mm aggregates and showed a tendency similar to what the aggregates did in variation from treatment to treatment.It was also discovered that it is feasible to determine soil BRSP using the whole soil passed through 1 mm sieve,because both T-BRSP and EE-BRSP in the whole soil are highly correlated not only with those in 1~2 mm aggregates,but also with SOC and TN in the soil.
Key words:  Long-term fertilization  Aggregate particle size  BRSP  Soil