石油污染土壤的生态风险评价和生物修复Ⅳ.油泥的预制床修复及其微生物群落变化
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中国科学院知识创新工程重要方向项目(KSCX2-YW-G-053-3)、国家高技术研究发展计划(863计划)(2009AA06Z402)和中国科学院南京土壤研究所知识创新工程领域前沿项目(ISSASSIP0724)资助


Eco-risk assessment and bioremediation of petroleum Ⅳ. Prepared bed remediation of oily sludge and changes in microbial community during remediation
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    摘要:

    通过添加有机肥、调理剂、接种烃降解菌以及搭建温室等方式对某油田的含油污泥进行了大规模的预制床生物修复。结果表明,经过230 d的修复,不同修复处理油泥中的油和脂含量降低了27.5%~46.3%,其中处理RCPG(有机肥+调理剂+接种烃降解菌+温室)的修复效率最高,而在对照中仅为15.1%。经统计分析,在所有处理措施中添加有机肥、调理剂以及搭建温室对修复效率具有显著影响(p< 0.05),而投加菌剂效果不显著。生物修复措施在降低油泥中污染物含量的同时也改善了油泥的理化性质,使氮、磷等营养物含量及田间持水率显著升高。同时结果还表明,所有修复处理油泥中的烃降解菌数量、微生物活性与多样性与对照相比均有极显著增加(p<0.01)。

    Abstract:

    Field-scale bioremediation of oily sludge on prepared beds was studied at the Shengli Oilfield in North China. Effects of addition of manure or soil conditioners (coarse sand and sawdust), inoculation of hydrocarbon degrading bacteria, and setting up greenhouses on oil and grease removal rate were evaluated. After 230 days of bioremediation, oil and grease content fell by 31.5~41.8 g kg-1 dry sludge in treated plots, indicating a removal rate ranging from of 27.5% to 46.3% compared with that in the control plot, being only 15.1%. Addition of manure or soil conditioners and setting up of greenhouses significantly increased the removal rate (p < 0.05). Moreover, the physic-chemical properties of the sludge in all treatment plots improved significantly after bioremediation. Microbial biomass in sludge and community-level physiological profiling (CLPP) analyzed using BIOLOG microplates were also studied. Total petroleum hydrocarbon(TPH)degraders and polycyclic aromatic hydrocarbon (PAH) degraders increased in all treated oily sludge. The activity of sludge microbial communities, characterized by average well color development (AWCD) value and Shannon diversity index, increased markedly in the treatment plots compared with that in the control.

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刘五星,骆永明,余冬梅,滕应,李振高.石油污染土壤的生态风险评价和生物修复Ⅳ.油泥的预制床修复及其微生物群落变化[J].土壤学报,2010,47(4):621-627. DOI:10.11766/trxb200808290405 Liu Wuxing, Luo Yongming, Yu Dongmei, Teng Ying, Li Zhengao. Eco-risk assessment and bioremediation of petroleum Ⅳ. Prepared bed remediation of oily sludge and changes in microbial community during remediation[J]. Acta Pedologica Sinica,2010,47(4):621-627.

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