Morphological, cultural and physio-biochemical traits and 16S rRNA sequence was analyzed of a strain of actinomycete, separated from the soil of the Zijin Mountain in Nanjing, China and coded as CT205, for identification and orientation in the classification system. The PDA agar plate method was used to determine antifungal activity of Strain CT205 and a pot experiment using pathogenic soil from a field that had been cultivated with cucumber consecutively for year was conducted to investigate the effect of Stain CT205 controlling cucumber Fusarium wilt. Based on the 16S rRNA sequence analysis, Strain CT205 was tentatively identified as Streptomyces albospinus (99% identity). And the pot experiment showed that the strain inhibited quite effectively Fusarium oxysporum f. sp. cucumerinum, Fusarium oxysporum f. sp. niveum, and Phytophthora nicotianae, and to a varying extent, Bacillus subtillus, Saccharomyces cerevisiae, Rhizoctonia cerealis, Ralstonia solanacearum. Besides its cucumber Fusarium wilt controlling efficiency being 51.85%, the strain promoted somewhat the growth of cucumber. When the strain was prepared with organic manure into complex biomanure, the disease controlling efficiency may reach as high as 81.85%. The findings demonstrate that the strain has certain potential application value.