水稻根系内生细菌对未来大气CO2浓度升高的响应
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科技部国际合作专项项目“大气组成变化及其影响与对策研究”(2010DFA22770)和中国科学院应用微生物研究网络项目(KSCX2-EW-G-16)资助


Response of endophytic bacteria in rice roots to elevated CO2
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    摘要:

    针对中国FACE(Free Air CO2 Enrichment)平台的镇籼96、扬稻8号、II优084和扬两优6号四种水稻品种,采用新一代高通量测序技术,研究了水稻根系内生菌的整体微生物群落对未来大气CO2浓度升高的响应。结果表明,水稻内生菌群落中γ-变形菌纲的肠杆菌科相对丰度最高,占整体微生物群落的30.8%~59.8%。对于镇籼96、扬稻8号和II优084三种水稻品种,大气CO2浓度升高可能抑制了数量上占优势的微生物菌群(优势菌群)生长,而促进了数量上不占优势的微生物菌群(稀少菌群)繁殖。例如,对于II优084品种,相对丰度高于14.6%的4种水稻内生菌为肠杆菌科、假单胞菌科、黄单胞菌科和气单胞菌科,大气CO2浓度升高,这些优势菌群的相对丰度之和由74.8%降为67.2%;相反,稀少菌群主要由鞘脂杆菌科、丛毛单胞菌科、黄杆菌科及草酸杆菌科组成,其相对丰度之和则由4.13%增至16.9%,其中,与对照相比,鞘脂杆菌科相对丰度增加比例高达344倍,是大气CO2浓度升高的最敏感微生物类群。但对于水稻品种扬两优6号,根系内生菌对大气CO2浓度升高的响应模式与其他三种品种不完全一致。这些研究结果表明,微生物的相对丰度可能是影响水稻根系内生菌对大气CO2浓度升高响应的重要因素,为研究全球变化下整体微生物结构与功能的演变规律提供了一定的依据。

    Abstract:

    Using the new high-throughput sequencing technique, study was carried out on responses to elevated CO2 (e CO2) of endophytic bacteria at the whole microbial community level in the roots of ZhenXian-96 (ZX-96), YangDao-8 (YD-8), II You-084 (TY-084), and YangLiangYou-6 (YLY-6), commonly used in the FACE experiment in China. Results show that the family of Enterobacteriaceae within the class of γ-proteobacteria was the highest in relative abundance, accounting for 30.8%~59.8% of the whole community. In ZX-96, YD-8, and TY-084,e CO2 would probably inhibit growth of the microbial groups that are dominant in population (dominant groups) but stimulate growth of those that are less (rare groups). For instance, in rice TY-084, Enterobacteriaceae, Pseudomonadaceae, Xanthomonadaceae, and Aeromonadaceae were the four dominant bacterial groups, of which each exceeded 14.6% in relative abundance, and their total relative abundance declined from 74.8% to 67.2% under e CO2. On the contrary, the rare groups in the roots, consisting of Sphingobacteriaceae, Comamonadaceae, Flavobacteriaceae, and Oxalobacteraceae, increased from 4.13% to 16.9% in total. Especially the family of Sphingobacteriaceae increased by up to 344 folds, and hence is the microbial group the most sensitive to e CO2. However, in YLY-6, the responses of endophytic bacteria differed in pattern from those in other varieties of rice. These findings indicate that relative abundance of microbes may probably be an important factor affecting the response of endophytic bacteria in the roots to elevated CO2, which may be used as certain basis for the study on variation of structure and function of the whole microbial community in response to the global climate change.

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任改弟,张华勇,林先贵,朱建国,贾仲君.水稻根系内生细菌对未来大气CO2浓度升高的响应[J].土壤学报,2013,50(6):1162-1169. DOI:10.11766/trxb201302060073 Ren Gaidi, Zhang Huayong, Lin Xiangui, Zhu Jianguo, Jia Zhongjun. Response of endophytic bacteria in rice roots to elevated CO2[J]. Acta Pedologica Sinica,2013,50(6):1162-1169.

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  • 收稿日期:2013-02-06
  • 最后修改日期:2013-04-12
  • 录用日期:2013-07-05
  • 在线发布日期: 2013-08-28
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