Illustrating mechanisms of N₂O generation in and emissions of CO₂ and CH₄ from the soil could help us design greenhouse gas mitigation strategies. An experiment was carried out in fields of acid red soil different in land use, i.e. vegetable garden, paddy field, tea garden and forest in Jinjing river region, Changsha, to study effects of application of carbon, nitrogen and nitrification inhibitor on N₂O, CO₂ and CH₄ emissions under constant temperature and soil moisture, using the static incubation-gas chromatograph method. Results show that less N₂O emission was observed from the acid red soil, low in pH, after application of N fertilizer; the addition of glucose stimulated N₂O emission from the soil applied with urea and from soil denitrification. Heterotrophic nitrification might be the main pathway of N₂O generation in acid red soil and nitrification inhibitor Dicyandiamide (DCD) had no significant effect on N₂O reduction in the acid red soil. In terms of total N₂O emission from the soil applied with N and C, the fields different in land use followed the the order of tea garden > vegetable garden > paddy field > forest land. Extraneous organic carbon could significantly stimulate soil CO₂ emission from the four fields, showing an order of tea garden and paddy field > vegetable garden and forest land, but did not have mucheffect on CH₄ emission in all the four fields except paddy field.