褚冰杰,余光辉,刘飞飞,沈其荣,冉 炜.土壤微团聚体中矿物-有机复合体特征[J].土壤学报,2017,54(6):1451-1458. CHU Bingjie,YU Guanghui,LIU Feifei,SHEN Qirong,RAN Wei.Characterization of Mineral-organic Complex in Soil Microaggregates with Synchrotron Radiation Infrared Micro-imaging Method[J].Acta Pedologica Sinica,2017,54(6):1451-1458 本文二维码信息
二维码(扫一下试试看!)
土壤微团聚体中矿物-有机复合体特征
Characterization of Mineral-organic Complex in Soil Microaggregates with Synchrotron Radiation Infrared Micro-imaging Method
投稿时间:2017-01-08  修订日期:2017-06-19
DOI:10.11766/trxb201704270011
中文关键词:  土壤团聚体  黏土矿物  有机物  矿物-有机复合体  同步辐射红外显微成像
Key Words:Soil aggregate  Clay minerals  Organics  Mineral-organic complex  Synchrotron radiation infrered micro-imaging technique
基金项目:国家自然科学基金面上项目(41371248)和国家重点研发计划重点项目(2017YFD0800803)
作者单位E-mail
褚冰杰 南京农业大学资源与环境科学学院江苏省固体有机废弃物资源化高新技术研究重点实验室 2015103135@njau.edu.cn 
余光辉 南京农业大学资源与环境科学学院江苏省固体有机废弃物资源化高新技术研究重点实验室 yuguanghui@njau.edu.cn 
刘飞飞 南京农业大学资源与环境科学学院江苏省固体有机废弃物资源化高新技术研究重点实验室 1468638287@qq.com 
沈其荣 南京农业大学资源与环境科学学院江苏省固体有机废弃物资源化高新技术研究重点实验室 shenqirong@njau.edu.cn 
冉 炜 南京农业大学资源与环境科学学院江苏省固体有机废弃物资源化高新技术研究重点实验室 ranwei@njau.edu.cn 
摘要点击次数: 478
全文下载次数: 854
中文摘要:
      以国家肥力网湖南祁阳红壤长期定位试验站的长期施有机肥和长期施化肥的土壤为研究对象,采用干筛法获得土壤微团聚体。利用同步辐射红外显微成像法研究土壤微团聚体中黏土矿物和有机官能团的原位分布图谱及其相关性。结果表明:土壤中黏土矿物(3 620 cm−1)和大分子有机物(脂肪,2 920 cm−1;蛋白质,1 650 cm−1;多糖,1 080 cm−1)呈高度异质性的分布规律。其中,黏土矿物和多糖有较为相似的分布模式;而黏土矿物和脂肪、蛋白类物质则呈现差异较大的分布模式。与长期施化肥处理的土壤微团聚体相比,长期施有机肥处理的土壤微团聚体中黏土矿物和大分子有机物呈现更高的分散性。此外,施有机肥和化肥处理土壤微团聚体样品中黏土矿物与有机官能团的决定系数(R2)均为:黏土矿物-脂肪 > 黏土矿物-多糖 > 黏土矿物-蛋白质,表明土壤微团聚体中黏土矿物和大分子有机物的亲和性有差异,且该差异不受长期施肥处理的影响。同步辐射微区域红外谱进一步表明,从土壤微团聚体外部到内部,黏土矿物的特征峰和大分子有机物的特征峰强度均逐渐增加。
Abstract:
      【Objective】In this study, the synchrotron radiation infrared micro-imaging method was used to characterize mineral-organic complex in soil microaggregates of the red soils under long-term application of organic manure or chemical fertilizer in the long-term stationary experiment station of the national soil fertility network in Qiyang, Hunan, China. 【Method】Soil samples were collected from the long-term experiment station for fractionation of soil microaggregates using the dry sieving method. The obtained soil microaggregates were spread over on a Whatman GF/A Filter, mounted onto a sieve and fixed to a chimney funnel that transferred warm mist from a humidifier filled with ultrapure water. Excess droplets on the filter were drained. Then the soil microaggregates were frozen at -20 ℃ and directly cut into thin sections (300~600 nm) on an ultramicrotome using a diamond knife. Sections were transferred to infrared-reflecting MirrIR Low-E microscope Slides. 【Result】Results show that clay minerals (3 620 cm-1) and larger molecular organics (i.e., lipids, 2 920 cm-1; proteins, 1 650 cm-1; and polysaccharides, 1 080 cm-1) were distributed in a pattern highly heterogeneous. However, among them clay minerals and polysaccharides were quite similar in distribution pattern, but differed sharply from lipids and proteins. Compared to the soil under long-term chemical fertilization, the soil under long-term application of organic manure had clay minerals and larger molecular organics distributed in soil microaggregates with higher dispersivity. Furthermore, in terms of determination coefficient (R2) with clay minerals, the organic functional groups followed an order of lipid > polysaccharide > protein in both soils, suggesting that affinity of clay minerals with larger molecular organics varied in soil microaggregates, and was not affected by fertilization of either chemical or organic. Meanwhile, the synchrotron radiation microzone infrared spectra further demonstrated that characteristic peaks of both clay minerals and larger molecular organics increased in intensity from the outer to the inner of the soil microaggregates. 【Conclusion】In summary, the synchrotron radiation infrared micro-imaging method provides a novel research means and useful information to the study on characterization of mineral-organic complexes in soil microaggregates.
查看全文  查看/发表评论  下载PDF阅读器