引用本文:辜夕容,倪亚兰,江亚男,贾 豪,何新华.接种双色蜡蘑对马尾松根际土壤无机磷和 活性铝含量的影响[J].土壤学报,2018,55(5):1179-1189.
GU Xirong,NI Yalan,JIANG Yanan,JIA Hao,HE Xinhua.Effect of Laccaria Bicolor Inoculation on Contents of Inorganic Phosphorus and Labile Aluminum in the Rhizosphere Soil of Pinus massoniana Saplings[J].Acta Pedologica Sinica,2018,55(5):1179-1189
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接种双色蜡蘑对马尾松根际土壤无机磷和 活性铝含量的影响
辜夕容
西南大学资源环境学院;西南大学土壤生物研究中心
摘要:
将双色蜡蘑(Laccaria bicolor)的3个株系(Lb 270、LbS238A和Lb S238N)分别与马尾松种子拌匀,培养在酸性土壤中,以检测根际土壤机磷和活性铝含量,并分析外生菌根对磷有效性和铝活性的作用。结果发现,与非菌根苗相比,接种双色蜡蘑显著促进马尾松幼苗生长和对磷、铝的吸收,而对根际土壤机磷和活性铝含量的影响因菌株而异:Lb 270和Lb S238N显著降低pH、有效磷、全铝、交换态铝含量,提高羟基态铝含量,且Lb270还显著降低铁磷和钙磷含量;Lb S238A显著提高pH、有效磷含量,减少铝磷、全铝、活性铝、交换态铝和腐殖酸铝含量。即接种双色蜡蘑提高了磷的生物有效性,且Lb 270和Lb S238N增加、Lb S238A减少铝的活性。因此,接种供试双色蜡蘑都能提高马尾松的抗铝性,主要在于其增加磷的生物有效性,而是否降低铝的活性取决于菌株特性。
关键词:  活性铝  铝毒害  外生菌根  机磷  马尾松
DOI:10.11766/trxb201802120407
分类号:
基金项目:国家自然科学基金项目(31570599、31100464)
Effect of Laccaria Bicolor Inoculation on Contents of Inorganic Phosphorus and Labile Aluminum in the Rhizosphere Soil of Pinus massoniana Saplings
Gu Xirong
College of Natural Resources and Environment, Southwest University; Centre of Excellence for Soil Biology, Southwest University
Abstract:
【Objective】 In most soils, TP (total phosphorus) is generally plenty, but its availability to plants is very low. When the soil is getting acidified, soil Al is liable to get activated and then bound with HPO42-,H2PO4-, and PO4-, turning them into hard-to-dissolve P, thus further lowering soil P availability. Some ectomycorrhizae (EM) are found to be able to deactivate soil Al, thus letting Al-complexes release bonded P in available form, for plants to take up. That is to say, some EMs could be introduced into acid soils to improve soil P availability and plant growth. In experiments of in vitro cultivation of EM fungi, some demonstrated their ability to raise P availability in soils neutral or slightly acidic, but little has been found in the literature validating such ability in the field. The present study aimed to assess effects of inoculation of EM fungi on inorganic P availability and Al activity in the rhizosphere soil of saplings planted in acid soil, and to explore mechanism of EM colonization improving host plants' resistance to Al toxicity. 【Method】 Germinating Pinus massoniana seeds, inoculated with three strains of Laccaria bicolor, namely Lb 270, Lb S238A, and Lb S238N, separately, were imbedded into pots of cold sandy yellow soil collected from a P. massoniana stand, and irrigated twice a day to maintain a proper moisture regime for 16 weeks under the natural conditions. At the end, comparison was made of Pinus massoniana saplings inoculated with EM fungi with those in the control (without inoculation). 【Result】 Results show that inoculation of L. bicolor significantly affected the contents of inorganic P and labile Al in the rhizosphere soil of the saplings and the effects varied with the strain. Compared with the control, inoculation with any of the three L. bicolor strains significantly improved growth and P and Al, uptake of the saplings. In terms of the effect, the three strains exhibited an order of Lb S238N > = Lb 270 > Lb S238A. Inoculation with Lb270 significantly decreased soil pH, the contents of available P, iron-bound P (Fe-P), calcium-bound P (Ca-P), total Al, and exchangeable Al, and the proportion of exchangeable Al in the fraction of labile Al, but increased the proportion of aluminum-bound P (Al-P) in the fraction of inorganic P, and the contents of labile Al or hydroxyl-Al and their proportions in total Al. Inoculation with Lb S238A significantly reduced Al-P, the proportion of Al-P or Fe-P in the fraction of inorganic P, total Al, labile Al, exchangeable Al, organic-complexed Al, and the proportion of exchangeable Al and organic-complexed Al in the fraction of labile Al, but raised soil pH, the content off available P, the proportion of occluded-P (O-P) in the fraction of inorganic P, the proportion of labile Al in total Al, and proportions of hydroxyl-Al or acid-soluble Al in the fraction of labile Al. Inoculation with LbS238N significantly decreased soil pH, the contents of available P, total Al, exchangeable Al, and the proportions of exchangeable Al and organic-complexed Al in the fraction of labile Al, but increased the content of hydroxyl-Al, the proportion of labile Al in total Al and that of hydroxyl-Al in the fraction of labile Al.【Conclusion】All the findings in this experiment demonstrate that inoculation with L. bicolor can improve the resistance of P.massoniana saplings to Al toxicity, which may be attributed to the ability of the fungi to enhance bio-availability of soil P, but their abilities to lower Al activity vary with strain of the fungi.
Key words:  Active aluminum  Aluminum toxicity  Ectomycorrhizae (EM)  Inorganic phosphate  Pinus massoniana Lamb.