引用本文:王娟娟,王 倩,姜爱霞,裴 振,王晓凤,郭笃发.黄河三角洲盐生植被演替对土壤真菌碳源代谢多样性的影响[J].土壤学报,2018,55(5):1264-1275.
WANG Juanjuan,WANG Qian,JIANG Aixia,PEI Zhen,WANG Xiaofeng,GUO Dufa.Effects of Succession of Halophytic Vegetation on Carbon Metabolism Diversity of Fungi in The Yellow River Delta[J].Acta Pedologica Sinica,2018,55(5):1264-1275
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黄河三角洲盐生植被演替对土壤真菌碳源代谢多样性的影响
王娟娟,王倩,姜爱霞,裴振,王晓凤,郭笃发
山东师范大学,山东师范大学,山东师范大学,山东师范大学,山东师范大学,山东师范大学
摘要:
探究土壤真菌碳源代谢水平与盐生植被演替之间的关系,是理清生态系统的内部结构和功能变化的重要步骤。采用Biolog-FF微孔板培养法,结合土壤理化性质分析,研究黄河三角洲地区不同盐生植被下土壤真菌的碳源代谢活性特征。结果表明,在0~20 cm土层,每孔平均吸光值(Average well color development,AWCD值)表现为白茅>罗布麻>柽柳>马绊草>光板地,20~40 cm土层则表现为白茅>马绊草>柽柳>罗布麻>光板地,即随盐生植被正向演替,土壤真菌碳源代谢活性显著提高(P<0.05);各样地土壤真菌群落的香农-威尔指数H丰富度指数S和辛普森指数D按演替顺序均有显著提高,同一样地不同土层中,除马绊草群落,其余样地的3种指数均表现为0~20 cm>20~40 cm,说明盐生植被正向演替过程改善了土壤中真菌的多样性和丰富度,提高了群落中常见物种的优势度。土壤理化性质与土壤真菌吸光值、真菌多样性指数相关性分析结果表明,全氮、碱解氮、土壤有机质、磷酸酶和过氧化氢酶显著促进了土壤真菌的碳源代谢活性,土壤含盐量则显著抑制真菌的碳源代谢活性(P<0.05);土壤主成分分析表明,纤维醇、鸟氨酸和D-甘露醇等是0~20 cm土层下土壤真菌利用的主要碳源,D-山梨醇、丙三醇和L-天冬氨酸等则是20~40 cm土层下土壤真菌利用的主要碳源,表明糖类、醇类和酸类是控制土壤真菌碳源代谢发生差异的主要碳源。土壤真菌碳源代谢水平与盐生植被演替关系的结果为黄河三角洲的生态恢复和利用奠定了理论基础。
关键词:  黄河三角洲  盐生植被  土壤真菌  功能多样性  Biolog-FF技术
DOI:10.11766/trxb201712200461
分类号:
基金项目:山东省自然科学基金项目(ZR2012DM013,ZR2015DM012)和山东省高等学校科技计划项目(J14LD02)
Effects of Succession of Halophytic Vegetation on Carbon Metabolism Diversity of Fungi in The Yellow River Delta
Wang Juanjuan,Wang Qian,Jiang Aixia,Pei Zhen,Wang Xiaofeng and Guo Dufa
Shandong Normal University,Shandong Normal University,Shandong Normal University,Shandong Normal University,Shandong Normal University,Shandong Normal University
Abstract:
【Objective】 As an important component of the soil microorganisms, fungi play an important role in cycling nutrients, maintaining and building soil fertility and improving soil structure and so on. As a decomposer in the soil, fungi can effectively decompose various kinds of macromolecule materials and hard-to-decompose litters in the soil, promote nutrient uptake of plants, and be used as an indicator of ecosystem health. Carbon source utilization capacity of soil fungi reflects overall metabolic characteristics of the fungal communities. To explore relationships between soil fungal carbon source metabolism and succession of halophytic vegetation is an important step to elucidate variation of the ecosystem in internal structure and function. 【Method】 In the region of the Yellow River Delta, in line with the natural succession of the halophytic vegetation, sample plots of bare coastal tidal flats, flats covered with Tamarix chinensis and Angiospermae (highly salt-tolerant plant community), and flats of Imperata and A.venetum (mildly salt-tolerant plant community) were selected, three each, making up a total of 15 sample plots for soil sampling in the 0~20 cm and 20~40 cm soil layers. The soil samples were analyzed for physicochemical properties, and carbon source metabolic activity of soil fungi using the Biolog-FF microplate culture method, and further for characterization of the carbon metabolic activity of the soil fungi in the plots relative to halophytic vegetation. 【Result】 The Biolog-FF microplate tests show that in terms of average well color development in the 0~20 cm soil layer, the plots followed an order of Imperata > A. venetum > Tamarix chinensis > Angiospermae > Bare plot, while in terms of AWCD in the 20~40 cm soil layer, they did an order of Imperata> Angiospermae > Tamarix chinensis > A. venetum > Bare plot, which suggests that with the succession of the halophytic vegetation, carbon metabolism activity of the fungi in the soil increases significantly (P) and so was Shannon-Wiener index, richness index and Simpson index of the fungi. In all the sample plots, except for that under Angiospermae, all the three indices were higher in the 0~20 cm soil layer than the 20~40 cm one, which suggests that with the succession of halophytic vegetation going on positively, the fungi in the soil improve in diversity and abundance and in dominance of common species in the community. Correlation analysis of soil properties, fungal AWCD and fungal diversity shows that TN, AN, SOM, phosphatase and catalase significantly (P<0.05) promoted while soil salinity significantly (P<0.05) inhibited fungal metabolism of carbon sources. Principal component analysis shows that Fibric alcohol, ornithine and D-mannitol were the main carbon sources for fungal utilization in the 0~20 cm soil layer, whereas D-sorbitol , glycerol and L-aspartic acid were in the 20~40 cm soil layer, which suggests that saccharides, alcohols and acids are the main carbon sources that control carbon metabolism of the fungi in the soil. 【Conclusion】 As a whole, with the succession of the halophytic vegetation going on positively, the soil underneath declines gradually in salinization degree and improves in soil quality, and the fungal community in the soil is growing more and more stable in structure. All the findings about relationships between carbon source metabolism of soil fungi and succession of halophytic vegetation may lay down a theoretical foundation for the ecological restoration and utilization of the Yellow River Delta.
Key words:  Yellow River Delta  Halophytic vegetation  Soil fungi  Functional diversity  Biolog-FF method