引用本文:张 倩,贺明荣,陈为峰,代兴龙,王振林,董元杰,诸葛玉平.外源一氧化氮与水杨酸对盐胁迫下小麦幼苗生理特性的影响[J].土壤学报,2018,55(5):1254-1263.
ZHANG Qian,HE Mingrong,CHEN Weifeng,DAI Xinglong,WANG Zhenlin,DONG Yuan-Jie,ZHUGE Yuping.Effects of Extraneous Nitric Oxide and Salicylic Acid on Physiological Properties of Wheat Seedlings under Salt Stress[J].Acta Pedologica Sinica,2018,55(5):1254-1263
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外源一氧化氮与水杨酸对盐胁迫下小麦幼苗生理特性的影响
张倩,贺明荣,陈为峰,代兴龙,王振林,董元杰,诸葛玉平
山东农业大学资源与环境学院,山东农业大学农学院,山东农业大学农学院,山东农业大学资源与环境学院,山东农业大学农学院,山东农业大学资源与环境学院,山东农业大学资源与环境学院
摘要:
在液培条件下,以小麦(山农22号)为试验材料,外源一氧化氮(Nitric oxide, NO)供体硝普钠(Sodium nitroprusside, SNP)和水杨酸(Salicylic acid, SA)作为调控物质,研究外源NO和SA单独及复配施用对 120 mmol•L-1 NaCl 胁迫下小麦生长及生理特性的影响。结果表明,120 mmol•L-1 NaCl胁迫严重抑制了小麦幼苗的生长,添加适宜浓度的SNP(100 μmol•L-1)或SA(100 μmol•L-1)均能显著缓解盐胁迫对小麦造成的伤害。而与单独添加SNP或SA相比,SA+SNP复合调控更能明显降低盐胁迫诱导的活性氧(Reactive oxygen species, ROS)积累、丙二醛(Malondialdehyde, MDA)含量以及电解质渗出率;提高小麦叶绿素含量、抗氧化酶活性和脯氨酸含量,从而提高其抗盐性;通过提高根系活力来促进对矿质元素的吸收,从而提高小麦幼苗的干物质积累;同时抑制了小麦对Na的吸收,以此减缓盐胁迫的毒害。由此说明NO与 SA在缓解小麦幼苗盐胁迫中表现出积极的协同作用。试验各处理中施用50 μmol•L-1SA+50 μmol•L-1 SNP的处理缓解小麦盐胁迫的效果最为明显。
关键词:  盐胁迫  一氧化氮  水杨酸  协同作用
DOI:10.11766/trxb201801230054
分类号:
基金项目:国家重点研发计划项目(2017YFD0201705)、国家重点基础研究发展计划项目(2015CB150404)和山东省自主创新及成果转化专项(2014ZZCX07402)
Effects of Extraneous Nitric Oxide and Salicylic Acid on Physiological Properties of Wheat Seedlings under Salt Stress
zhangqian,hemingrong,chenweifeng,daixinglong,wangzhenlin,DONG Yuan-Jie and zhugeyuping
Shandong Agricultural University,Shandong Agricultural University,Shandong Agricultural University,College of Agronomy Shandong Agricultural University,Shandong Agricultural University,Shandong Agricultural University,Shandong Agricultural University
Abstract:
【Objectives】Wheat is one of the most important crops cultivated in coastal saline soils. The seed germination period and seedling stage are two important growth stages in the life circle of wheat and the plants during the two stages are pretty sensitive to salt stress. This study was designed to explore effects of extraneous nitric oxide (NO) and/or salicylic acid (SA) applied alone or in combination, on growth and physiological properties of wheat under the stress of 120 mmol•L-1 NaCl in an attempt to evaluate the effects of extraneous NO and SA mitigating salt stress on growth of wheat seedlings, and to provide a theoretical basis for elucidating the mechanism of extraneous NO and SA enhancing salt tolerance of wheat. 【Methods】A hydroponic experiment was carried out, cultivating wheat (Triticum aestivum L.) and using extraneous sodium nitroprusside (SNP, a nitric oxide [NO] donor) and SA as regulatory substance to explore effects of extraneous NO and/or SA on growth and physiological properties of wheat under salt stress (120 mmol•L-1 NaCl). This experiment was designed to have six treatments; i.e. CK (Hoagland nutrient solution), NaCl (120 mmol•L-1NaCl), SA (120 mmol•L-1 NaCl+100 μmol•L-1 SA), SNP (120 mmol•L-1 NaCl+100 μmol•L-1 SNP),1/2 (SA+SNP) (120 mmol•L-1 NaCl+50 μmol•L-1 SA+50 μmol•L-1 SNP, and SA+SNP (120 mmol•L-1 NaCl+100 μmol•L-1 SA+100 μmol•L-1 SNP). Growth indices, root activity, photosynthetic pigment content, superoxide anion (O2•-) production rate, H2O2 content, MDA content, electrolyte leakage and antioxidase activities of the wheat seedlings in all the treatments were analyzed for comparison to explore effects of NO and/or SA. 【Results】Results show that Treatment NaCl was significantly higher in O2-1 production rate and H2O2content, but significantly lower in wheat growth and photosynthetic pigment synthesis. Treatment SNP or SA significantly mitigated the damage of wheat caused by salt stress, but the effect of Treatment SA+SNP was more significant. Compared with Treatment NaCl, Treatment 1/2(SA+SNP) was 74.26% higher in root activity and 39.58%, 24.46% and 33.06% higher in content of Chl a, Chl b and Car respectively, but 30.52% and 29.16% lower in O2-1 production rate in leaves and roots and 15.78% and 24.23% lower in content of MDA in leaves and roots, respectively. Besides, Treatment 1/2(SA+SNP) also significantly increased the content of antioxidase as well as the absorption of mineral elements, such as N, P, K and Ca. 【Conclusion】Compared with Treatment SNP or SA, Treatment SA+SNP is more effective in reducing ROS accumulation induced by salt stress, MDA content and electrolyte leakage, improving chlorophyll content, proline content and soluble protein content, antioxidase activity; enhancing root activity in absorbing mineral elements, as well as in inhibiting Na uptake and reducing Na content in the plants, thus relieving salt stress of the wheat seedlings. Among the treatments in the experiment, Treatment 1/2(SA+SNP) is the most effective in mitigating salt stress on wheat.
Key words:  Salt stress  Nitric oxide  Salicylic acid  Synergistic effect