引用本文:陈秀波,朱德全,赵晨晨,张路路,陈立新,段文标.不同林型红松林土壤真菌群落组成和多样性[J].土壤学报,2019,56(5):1221-1234.
CHEN Xiubo,ZHU Dequan,ZHAO Chenchen,ZHANG Lulu,CHEN Lixin,DUAN Wenbiao.Community Composition and Diversity of Fungi in Soils under Different Types of Pinus koraiensis Forests[J].Acta Pedologica Sinica,2019,56(5):1221-1234
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不同林型红松林土壤真菌群落组成和多样性
陈秀波1, 朱德全2, 赵晨晨2, 张路路2, 陈立新1, 段文标1
1.东北林业大学林学院;2.佳木斯大学理学院
摘要:
采用基于ITS(Internal Transcribed Spacer)序列的Illumina MiSeq高通量测序技术,分析凉水国家级自然保护区的云冷杉红松林、椴树红松林、红松人工林和红松天然次生林四种林型的土壤真菌群落组成和多样性。共获得1 316个OTUs,四种林型的土壤含有大量未知真菌,在已知真菌类群中,子囊菌门(Ascomycota)和担子菌门(Basidiomycota)为优势菌门,其中后者在四种林型土壤中的分布差异显著(P<0.05)。α多样性分析结果表明:原始红松林(云冷杉红松林和椴树红松林)真菌群落丰富度指数(Ace和Chao1指数)显著高于红松天然次生林(P<0.05)。β多样性分析显示:四种林型土壤真菌群落组成差异显著(r=0.537,P=0.001),但两种原始红松林之间、人工林和天然次生林之间组成无显著差异。土壤全氮、总有机碳、pH、水分含量和总孔隙度是显著影响四种林型土壤真菌群落组成的环境因子(P<0.05)。研究结果为原始红松林的保护、人工植被恢复和真菌资源的开发利用提供依据。
关键词:  原始红松林  人工林  天然次生林  真菌群落结构  多样性
DOI:10.11766/trxb201810290533
分类号:
基金项目:国家自然科学基金项目(31670627,31770656)和黑龙江省省属本科高校基本科研业务费项目(2017-KYYWF-0597)
Community Composition and Diversity of Fungi in Soils under Different Types of Pinus koraiensis Forests
CHEN Xiubo1, ZHU Dequan2, ZHAO Chenchen2, ZHANG Lulu2, CHEN Lixin1, DUAN Wenbiao1
1.College of Forestry, Northeast Forestry University;2.College of Science, Jiamusi University
Abstract:
【Objective】To investigate community composition and diversity of soil fungi in relation to type of Pinus koraiensis forest, a field survey was carried out in the Liangshui National Nature Reserve, where there are four types of Pinus koraiensis forest, that is, Picea koraiensis and Abies fabri-Pinus koraiensis forest, Tilia amurensis-Pinus koraiensis forest, Pinus koraiensis plantations and Pinus koraiensis secondary forest.【Method】Soil samples were collected under the four types of Pinus koraiensis forests for analysis by means of ITS (Internal Transcribed Spacer) sequence based Illumina miseq high-throughput sequencing.【Result】Results show that out of the 16 soil samples collected from the soils under the four types of Pinus koraiensis forests, a total of 505 299 high quality sequences and 534 359 effective sequences and a total of 1316 fungal OTUs were obtained. In the soils of the four types of forests, besides a huge number of unidentified fungi, Ascomycota and Basidiomycota were found to be in dominancy. Distribution of the latter varied from soil to soil (P<0.05).In the soils of the four types of forests, besides a huge number of unidentified fungi, Ascomycota and Basidiomycota were found to be in dominancy.There were significant differences in the distribution of Basidiomycota in the four forest types (P<0.05).And at the genus level, Leptodontidium, Simplicillium, Sebacina, Russula, Penicillium , and Cryptococcus were the dominant ones.The LDA scores of LEfSe analysis show that there were 19 fungi genera with significantly different abundance in four forest types, among 10 genera in the soil under Tilia amurensis-Pinus koraiensis forest and one genera in the soil under natural Pinus koraiensis secondary forest. Alpha diversity analysis shows that the soils under primitivePinus koraiensis forests (Picea koraiensis and Abies fabri-Pinus koraiensis forest and Tilia amurensis-Pinus koraiensis forest) were significantly higher than that under Pinus koraiensis secondary forest in fugal community richness index (Ace and Chao1 index). Beta diversity analysis shows that the soils differed significantly in fungi community composition (r=0.537, P=0.001), but no much difference was found between the two primitive Pinus koraiensis forests and between the Pinus koraiensis plantation and natural Pinus koraiensis secondary forest. Soil pH, moisture content, total porosity, total nitrogen and total organic carbon were the main factors influencing soil fungal community composition in the four forest soils (P<0.05).Correlation analysis of the top 20 dominant genus of fungi and soil physicochemical factors shows that 6 genera were significantly related to soil total nitrogen in relative abundance. Among the six genera, Russula, Suillus and unclassified-f-Thelephoraceae were positively related while Malassezia , Simplicillium and Penicillium were negatively.【Conclusion】There were significant differences in the composition of soil fungal community composition of four types of Pinus koraiensi forests, but there was no statistically difference in the composition of between the two primitive Pinus koraiensis forests and between the Pinus koraiensis plantation and natural Pinus koraiensis secondary forest. Soil total nitrogen may be the main factor affecting the composition of soil fungal communities of four forest types, followed by total organic carbon. All the findings in this study may serve as a theoretical basis for protection of primitive Pinus koraiensis forests, artificial rehabilitation of vegetation and exploitation of the fungal resources in the soil.
Key words:  Primitive Pinus koraiensis forest  Pinus koraiensis plantation  Natural Pinus koraiensis secondary forest  Soil fungal community structure  Diversity