引用本文:肖玖军,邢 丹,毛明明,王 岩.AM真菌对桑树根围土壤团聚体的影响机制[J].土壤学报,2020,57(3):773-782. DOI:10.11766/trxb201903170534
XIAO Jiujun,XING Dan,MAO Mingming,WANG Yan.Mechanism of arbuscular mycorrhizal fungal affecting soil aggregates in rhizosphere of mulberry (Morus alba)[J].Acta Pedologica Sinica,2020,57(3):773-782. DOI:10.11766/trxb201903170534
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AM真菌对桑树根围土壤团聚体的影响机制
肖玖军1, 邢丹2, 毛明明3, 王岩2
1.贵州省山地资源研究所;2.贵州省农科院蚕业研究所;3.贵州大学农学院
摘要:
为揭示丛枝菌根 (Arbuscular mycorrhizal,AM)真菌对植桑土壤的影响及机制,采用盆栽试验研究接种摩西管柄囊霉 (Funneliformis mosseae)和根内根生囊霉 (Rhizophagus intraradices)对土壤有机碳(Soil organic carbon, SOC)、球囊霉素相关土壤蛋白 (Glomalin related soil protein, GRSP)及团聚体组成与稳定性的影响。结果表明:⑴ 接种R. intraradices显著增加土壤大团聚体百分比,并提高平均质量直径 (Mean weight diameter, MWD)和几何平均直径 (Geometric mean diameter, GMD)、显著降低团聚体破坏率 (Percentage of aggregate destruction, PAD)。⑵ 接种F. mosseaeR. intraradices均显著增加微团聚体SOC含量,接种F. mosseae显著降低大团聚体总GRSP含量,而接种R. intraradices却显著增加大团聚体和微团聚体总GRSP含量及易提取GRSP含量。⑶ 接种AM真菌对整体SOC的效应为负,土壤总GRSP对SOC占比在25.5%~76.5%之间,土壤易提取GRSP对SOC占比在4.87%~5.93%之间,且R. intraradices的接种效应高于F. mosseae。⑷ 总GRSP、易提取GRSP和SOC对团聚体组成表现均为正向显著影响,其中易提取GRSP是主要驱动因子,而总GRSP是土壤团聚体稳定性的主要影响因子。综上,AM真菌作用下桑树根围土壤团聚体得以改善并趋于稳定,R. intraradices的接种效应明显大于F. mosseae;土壤团聚体的形成主要依赖易提取GRSP,而其稳定性主要受总GRSP影响。
关键词:  丛枝菌根真菌  团聚体  球囊霉素相关土壤蛋白  土壤有机碳
基金项目:国家自然科学基金项目(31460225)和贵州省科技计划项目(黔科合平台人才[2018]5753)
Mechanism of arbuscular mycorrhizal fungal affecting soil aggregates in rhizosphere of mulberry (Morus alba)
xiaojiujun1, Xing Dan2, MAO Mingming3, Wang Yan2
1.Institute of Mountain Resources of Guizhou province;2.Sericultural Institute,Guizhou Academy of Agricultural Sciences;3.College of agriculture,Guizhou University
Abstract:
【Objective】 Soil aggregates, as basic units of the soil in structure, are of huge influence for water penetration and retention in soil, topsoil erosion and plant growth. Their stability is one of the main indicators in evaluation of soil anti-erodibility. Arbuscular mycorrhizal fungi (AMF) may adjust soil structure via influencing formation of soil macro-aggregates and stability of water-stable aggregates. To explore the effects and potential mechanisms of arbuscular mycorrhizal fungi (AMF) affecting formation and stability of soil aggregates in rhizosphere of mulberry, a pot experiment was carried out.【Method】In the experiment, mulberry trees were planted in the pots and the soil in the pots was inoculated with Funneliformis mosseae (F. mosseae) and Rhizophagus intraradices (R. intraradices), separately. After a certain period of cultivation, the soils in the pots were collected with the shaking off method, and separated into three aggregate fractions by particule size (0.25~5 mm,0.053~0.25 mm and <0.053 mm)using the dry and wet sieving method for analysis of composition and stability of soil aggregates, glomalin related soil protein (GRSP) and soil organic carbon (SOC) in the rhizosphere of mulberry to evaluate effects of the inoculation.【Result】 Results show that R. intraradices-inoculation expanded the fraction of macro-aggregates significantly in proportion, and increased mean weight diameter (MWD) and geometric mean diameter (GMD) of the aggregates and deceased their destruction rate (PAD), as compared to the control. Interestingly, F. mosseae-inoculation did not have much the effects. However, both F. mosseae- and R. intraradices-inoculations increased the content of SOC in micro-aggregates significantly. In addition, F. mosseae-inoculation reduced total GRSP (T-GRSP) significantly in macro aggregates, while R. intraradices-inoculatioin increased the content of T-GRSP and easily-extracted GRSP (EE-GRSP) significantly in both macro- and micro-aggregates. Inoculation of AMF had certain negative effects on overall SOC, of which T-GRSP accounted for 25.5%~76.5% and EE-GRSP did for 4.87%~5.93%, and the effects were more significant in R. intraradices-inoculted soils than in F. mosseae-inoculated ones. Moreover, the composition of soil aggregates was significantly and positively related to soil T-GRSP, EE-GRSP and SOC. Among them EE-GRSP was the main driving factor, and T-GRSP was the key factor affecting soil aggregate stability. 【Conclusion】 In short, all the findings suggest that AMF can significantly improve the composition and stability of soil aggregates in the rhizosphere of mulberry. R. intraradices was more effective than F. mosseae. The formation of soil aggregate depends mainly on EE-GRSP, while their stability is subject mainly to the influence of T-GRSP.
Key words:  Arbuscular mycorrhizal fungi  Aggregate  Glomalin related soil protein  Soil organic carbon