【Objective】 In order to expose influence of deep vertically rotary tillage (DVRT) technology on aggregate structure, and explore mechanism of the change, in-lab analyses of soil samples were carried out in this study. 【Method】 Soil samples were collected from farmlands of latosolic red soil different in tillage, conventional rotary tillage 20 cm deep (CT20), deep tilling 40 cm deep (DT40), DVRT 20cm deep (FL20) or DVRI 40 cm deep (FL40), were studied by means of aggregate analysis, scanning electron microscopy (SEM), brunauer-emmett-teller (BET) specific surface area analysis and pore size analysis for differences in morphology and structure of the soil before and after tillage and between the treatments of tillage. Changes in nutrient status and physicochemical properties of the latosolic red soil after tillage were analyzed and compared. 【Result】Results show that FL40 increased the content of mechanical stability aggregates, 1~0.25 mm in particle size (P<0.05) and decreased the content of water stability aggregates, >3 mm in particle size (P<0.05) as compared with other tillage methods. Compared with CT20 and DT40, FL (either FL20 or FL40) changed the soil in micro-morphology to have skeletal grains fine, closely arrayed and smooth in surface, and to be higher in soil specific surface area and more extensive in pore distribution. DT40 differed significantly from the other treatments in available nutrient content (P<0.05). 【Conclusion】Compared with CT20 and DT40, FL (either FL20 or FL40) can increase the content of aggregates in latosolic red soil, and changed the micromorphology of latosolic red soil significantly, and FL20 can increase crop yield significantly.