引用本文:漆婧华,王岩松,张雯莉,刘玉冰,李新荣,赵丽娜.沙坡头地区地衣和藓类结皮丛枝菌根真菌多样性研究[J].土壤学报,2020,57(4):986-994. DOI:10.11766/trxb201904190054
QI Jinghua,WANG Yansong,ZHANG Wenli,LIU Yubing,LI Xinrong,ZHAO Lina.AMF Diversity Analysis of Lichen and Moss Biocrusts in Shapotou Region[J].Acta Pedologica Sinica,2020,57(4):986-994. DOI:10.11766/trxb201904190054
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沙坡头地区地衣和藓类结皮丛枝菌根真菌多样性研究
漆婧华1,2, 王岩松1,2, 张雯莉1,2, 刘玉冰1, 李新荣1, 赵丽娜1,2
1. 中国科学院西北生态环境资源研究院,沙坡头沙漠试验研究站,兰州 730000;2. 中国科学院大学,北京 100049
摘要:
丛枝菌根真菌(Arbuscular mycorrhizal fungi, AMF)是自然界分布最广的一类植物共生真菌,可与生物土壤结皮(Biological soil crust, BSC)的隐花植物形成共生体,对荒漠植被的恢复和演替具有重要意义。目前针对不同类型BSC的AMF群落结构及其多样性研究较少,限制了AMF群落对BSC发育的作用和对荒漠生态系统贡献的理解。利用高通量测序技术,对腾格里沙漠东南缘沙坡头固沙植被区地衣和藓类结皮及其下层土壤AMF群落组成与多样性进行研究,分析不同结皮类型AMF群落结构的差异。结果发现,AMF群落丰富度和多样性表现为:藓类结皮下层土壤>地衣结皮下层土壤>藓类结皮>地衣结皮。地衣和藓类结皮及其下层土壤AMF均属于球囊菌门、球囊菌纲,以球囊霉属、类球囊霉属、盾巨孢囊霉属、巨孢囊霉属和一种未分类属为优势,且地衣和藓类结皮AMF群落在属水平差异显著。主成分分析结果显示,藓类结皮下层土壤中AMF群落物种组成与其他各组有显著差异,表明随着结皮的发育,AMF群落多样性增加,结皮演替与AMF群落多样性之间具有相互促进的作用。
关键词:  生物土壤结皮  丛枝菌根真菌  高通量测序技术  群落结构和多样性
基金项目:国家自然科学基金项目(41621001,41977204)
AMF Diversity Analysis of Lichen and Moss Biocrusts in Shapotou Region
QI Jinghua1,2, WANG Yansong1,2, ZHANG Wenli1,2, LIU Yubing1, LI Xinrong1, ZHAO Lina1,2
1. Shapotou Desert Research and Experimental Station, Northwest Institute of Eco-environment and Resource, Chinese Academy of Sciences, Lanzhou 730000, China;2. University of Chinese Academy of Sciences, Beijing 100049, China
Abstract:
【Objective】Arbuscular mycorrhizal fungi (AMF) are one of the most widely distributed plant symbiotic fungi in nature. They can form symbiont with cryptogam in biological soil crust (BSC), which is of great significance to the restoration and succession of desert vegetation. However, there are few studies on AMF community structure and diversity in different BSCs types, which limited the understanding of the roles of AMF community in the development of BSCs and its contribution to desert ecosystems. So, will the changes of BSC type affect the community characteristics of AMF complexes in desert ecosystems? 【Method】 In view of the problems mentioned above, this study was based on enclosed revegetation sites in the southeast fridge of the Tengger Desert located at the Shapotou Desert Research &Experiment Station, with the lichen and moss crusts and their underlying soils as the research object. We used high-throughput sequencing technologies studied the AMF community structure composition and diversity of lichen, moss crusts and their underlying soils, and to determine whether different BSCs types will cause differences in AMF community characteristics. 【Result】 The following results were obtained. AMF community richness and diversity index of the underlying soils of moss crusts were the largest and the underlying soils of lichen crusts were the smallest. The AMF community diversity of the underlying soils of moss crusts is significantly different from other groups. The richness and diversity of AMF community of underlying soils were higher than crusts layers, and moss crusts was higher than lichen cursts. The AMF in the lichen and moss crusts and their underlying soils consisted by Glomeromycetes, Glomeromycota. At the genus level,one unclassified species (g_unclassified_c_Glomeromycetes, 6.69%-99.99%) and Glomus (19.55%-47.18%), Paraglomus (0.01%-20.91%), Scutellospora (13.37%) and Gigaspora (7.72%) were the dominant species, and the species that could be classified into Diversisporaceae (g_unclassified_f_Diversisporaceae, 4.13%) were quite limited in percentage. The species of AMF community in the lichen crusts and their underlying soils were exactly the same in composition, but their relative abundance was different. Compared with lichen crusts and their underlying soils, only the species of Glomeromycetes and Glomus were found in the moss crusts, and the species of Paraglomus could not be found. Except for species that appeared in the other samples, there were also found an unclassified Diversisporaceae species and Paraglomus, Scutellospora, Gigaspora in the underlying soils of moss crusts. The species of the unclassified Glomeromycota and Glomus were coexisted in all samples, but the relative abundance was different, which indicates that they belonged to the dominant fungi in the BSCs of this area. The species of Glomus were found in the underlying soils of lichen and moss crusts, and the difference between them was not significant, but there were significant differences with lichen and moss crusts, which suggested that Paraglomus may be the plant symbiotic fungi in the surrounding environment. 【Conclusion】 With the development of BSCs, the diversity of AMF community increased, and the succession of BSC and diversity of AMF communities can promote each other.
Key words:  Biological soil crust  Arbuscular mycorrhizal fungi  Illumina MiSeq high-throughput sequencing technique  Community structure and diversity