引用本文:汪 欢,郑 越,杨烨怡,陈向南,杨 帆,吴雪娥,杨朝晖,赵 峰.湿地变形菌门甲烷氧化菌群的缺氧能量代谢[J].土壤学报,2020,57(4):1008-1016. DOI:10.11766/trxb201904040135
WANG Huan,ZHENG Yue,YANG Yeyi,CHEN Xiangnan,YANG Fan,WU Xue’e,YANG Zhaohui,ZHAO Feng.Energy Metabolism of Community Dominated by Proteobacteria Methanotroph in Anoxic Environment of Wetland[J].Acta Pedologica Sinica,2020,57(4):1008-1016. DOI:10.11766/trxb201904040135
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湿地变形菌门甲烷氧化菌群的缺氧能量代谢
汪 欢1, 郑 越2, 杨烨怡2, 陈向南2, 杨 帆2, 吴雪娥3, 杨朝晖1, 赵 峰2
1.湖南大学环境科学与工程学院,环境生物与控制教育部重点实验室;2.中国科学院城市环境研究所,中国科学院城市污染物转化重点实验室;3.厦门大学化学化工学院化学工程与生物工程系
摘要:
甲烷氧化菌以甲烷作为碳源和能源,在全球甲烷平衡和温室效应控制中扮演着重要角色。甲烷生物氧化过程跨越不同氧化还原生态位,近年来的研究表明,在湿地缺氧生态位下变形菌门甲烷氧化菌具有代谢潜力,但其能量代谢机制尚不清楚。本研究基于生物电化学技术、矿物学实验及微生物组学方法,结果表明变形菌门甲烷氧化菌主导的菌群具有直接和间接胞外电子传递潜力;在氧气耗尽时,甲烷氧化菌群可利用水铁矿作为电子受体完成能量代谢过程,缺氧体系中γ-Proteobacteria纲的甲烷氧化菌和非甲烷氧化微生物共同驱动铁矿还原。本研究探讨了变形菌门甲烷氧化菌主导菌群的缺氧能量代谢过程,拓展了反硝化厌氧甲烷氧化菌及厌氧甲烷氧化古菌主导的缺氧甲烷氧化理论,为甲烷生物控制提供了理论支持。
关键词:  甲烷氧化菌  变形菌门  胞外电子传递  缺氧生态位  微生物组学
基金项目:国家自然科学基金项目(21777155)和国家重点研发计划项目(2018YFC1800502)资助
Energy Metabolism of Community Dominated by Proteobacteria Methanotroph in Anoxic Environment of Wetland
WANG Huan1, ZHENG Yue2, YANG Yeyi2, CHEN Xiangnan2, YANG Fan2, WU Xue’e3, YANG Zhaohui1, ZHAO Feng2
1.Key Laboratory of Environmental Biology and Pollution Control, College of Environmental Science and Engineering, Hunan University;2.CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences;3.Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University
Abstract:
【Objective】 Methanotrophs, using methane as carbon and energy sources for growth, play an important role in keeping balance of global methane in balance and controlling greenhouse effects. The process of bio-oxidation of methane spans over different redox niches. Recent researches demonstrate that methanotrophs. Belonging to the phylum of Proteobacteria have the potential of methane metabolism in anoxic niches of wetland, however so far little has been reported on mechanisms of their energy metabolism. This study was to explore mechanism of the energy metabolism of the Proteobacteria dominated methanotrophs in anoxic niches from three aspects.【Method】Bioelectrochemical techniques were used to explore potential of extracellular electron transfer of the methanotrophs. In exploring for potential electrons of the methanotrophs, the reaction system, when aerobic, was designed to have two potential electron acceptors, i.e. oxgyen and ferrihydrite and when anoxic, only one, i.e. ferrihydrite to study energy metabolism of the methanotrophs in anoxic conditions. Mineralogy analysis of the ferrihydrite in the system was performed to determine reduction dynamics of the mineral and structure of its secondary mineral. And analyses of community composition of the methanotrophs before and after reduction of the ferrihydrite were conduction to determine changes in the community.【Result】Results show that the methanotroph groups were able to transfer directly or indirectly extracellular electrons. Once oxygen was used up, the methanotrophs could keep on their energy metabolism by making use of ferrihydrite as electron acceptor. In the anoxic condition, the methane-oxidizing bacteria could reduce the iron mineral 50 times as fast as the ANME (Anaerobic methanotrophic archaea) anoxic methanotrophic archaea, and the secondary mineral were tentatively found to be vivianite via SEM (Scanning electron microscope), EDS (Energy disperse spectroscopy) and XRD (X-ray diffraction) analysis. Based on the principal component analysis of the methanotroph groups, the microbial community varied in composition relative to mode of energy metabolism. Comparison of the methanotroph groups at the phylum level found that in the hyperoxic systems, γ-Proteobacteria in reduced ferrihydrite increased up to 56% in relative abundance, and Methylococcales became the only one species of methanotroph in phylum, while in the anoxic systems, γ-Proteobacteria decreased down to 6% in relative abundance, but α-Proteobacteria increased up to 31%. 【Conclusion】In the anoxic systems, methanotrophic bacteria (γ-Proteobacteria) and non-methanotrophic bacteria worked together driving iron reduction. This study has revealed the process of energy metabolism of the Proteobacteria dominated methanotroph groups, and developed the theory of methane oxidation driven by NC10 bacteria and ANME archaea in anoxic environment, and hence provide certain theoretic support to future studies on bio-control of methane prodction.
Key words:  Methanotrophs  Proteobacteria  Extracellular electron transfer  Anoxic niches  Microbial omics