[Objective] Longya Lily (Lilium brownii var. viridulum) is a perennial herb aboriginal of Longhui County, Shaoyang City, Hunan Province, with edible and medicinal values. It is of great significance to sustain cultivation of the crop for farmers' income and local economic development. However, soil borne diseases and weeds are the two key adverse factors affecting growth of replanted Longya Lily, and hence threatening stability and development of the local Lily industry. Reductive soil disinfestation (RSD) refers to a technology of pre-planting soil treatment designed to effectively eliminate soil-borne pathogens, degrade allelochemicals, and improve soil physicochemical properties, etc., but how much RSD could control weeds and soil weed seed bank is still unclear. Therefore, this study was oriented to explore effects of RSD on soil borne pathogens and weed seed bank in Longya Lily fields.[Method] A field experiment, designed to have three treatments, i.e. CK (control without soil treatment); MO[RSD incorporated with 6 t·hm^-2liquid organic material (C/N 21)]; SB[RSD incorporated with 15 t·hm^-2 solid organic material (C/N 94)], was conducted in a field planted with Longya Lily for one year. Real-time PCR was used to determine populations of the bacteria, fungi and soil-borne pathogens in the soil. Species of the weeds in the field were identified and density and biomass of the weeds were recorded.[Result] Results show that RSD effectively suppressed Fusarium oxysporum, Fusarium solani and Rhizoctonia solani, and significantly lowered the proportion of genus Fusarium in the fungal community as compared to CK, with disinfestation efficacy up to 98.8%. Furthermore, RSD also effectively inhibited germination of most of the weeds in the soil weed seed bank, and significantly reduced density and biomass of the weeds in the field, by 94.1%~96.0% and 71.0%~94.7%, respectively. And treatment MO was higher than treatment SB in weed control effect. In addition, RSD significantly changed structure and reduced diversity, richness and dominance of the weed community.[Conclusion] Therefore, it can be concluded that RSD can significantly reduce soil borne pathogens, weeds and other adverse factors in the field of replanted Longya Lily. It is a promising agricultural measure with the potential to reduce the occurrence of both soil borne diseases and weed infestation. The findings in this study could provide a theoretical basis and certain technical support for reducing the use of pesticide and realizing sustainable development of the green agriculture.