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种植模式影响施肥导致的土壤反硝化势变化及其微生物机制
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X17

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国家自然科学基金项目（41977102）和江西省自然科学基金项目（20192BAB203022）资助


Effect of Planting System on Fertilization-induced Variation of Soil Denitrification Potential and Its Microbial Mechanism
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Funding for the work was provided by the National Natural Science Foundation of China (41977102) and Natural Science Foundation of Jiangxi Province (20192BAB203022)

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    摘要:

    水田土壤反硝化势（Soil denitrification potential，SDP）往往高于旱地土壤，但施肥对水田和旱地SDP的影响差异往往基于不同气候条件下的不同土壤类型获取，其准确性可能受外界条件干扰。以发育自同一母质的相邻水田和旱地长期试验为平台，比较不同施肥模式下水田和旱地SDP的变化及其与功能基因（narG、nirS、nirK和nosZ）丰度及nirS-型反硝化细菌群落组成之间关系的异同。结果表明，在水田中，与常规氮磷钾平衡施肥（NPK处理）相比，缺钾（NP处理）和缺氮（PK处理）的SDP分别提高33.01%和23.57%，而缺磷（NK处理）则降低35.76%，其中NP和NK处理的SDP变化与nirS基因丰度显著相关。这可能与施肥导致的土壤有效磷和氮/磷变化有关，而PK处理的SDP变化与nirS-型反硝化细菌Azospira sp.NC3H-14丰度的显著升高有关。在旱地中，与NPK处理相比，NP、NK和缺氮钾的P处理SDP分别提高13.94%、26.51%和25.41%，NK和P处理的SDP变化既与narG基因丰度显著增加相关，也与不同的nirS-型反硝化细菌丰度增加有关，其中NK处理与Azospira sp.NC3H-14和Ideonella sp.NC3L-43b丰度增加有关，P处理与Azospira sp.NC3H-14、Rhodanobacter sp.D206a和Rubrivivax gelatinosus丰度增加有关；而土壤无定形氧化铁含量的变化可能是影响narG基因丰度的主要因素。直接比较相同环境条件下的水田和旱地结果可以发现，水田中施肥导致的SDP变化主要与反硝化微生物群落组成变化有关，而旱地中则可能同时受制于功能基因和反硝化微生物群落组成的变化。

    Abstract:

    [Objective] Soil denitrification potential (SDP) is generally higher in paddy field than in upland field. However, as the effect of fertilization on SDP in paddy field and upland field varies with climatic and soil type, accuracy of its assessment is often affected by external conditions.[Method] In this study, two adjacent fields, one paddy field and one upland field, both derived from the same parent material of Quaternary red clay, in a long-term field experiment were selected for exploration of effect of fertilization regime on SDP and its association with abundances of functional genes (narG, nirS, nirK, and nosZ) and community composition of nirS-type denitrifiers with the aid of in-lab incubation, real-time quantitative polymerase chain reaction (qPCR), and high-throughput sequencing technology.[Result] In the paddy field, compared with Treatment NPK, Treatment NP and PK was significantly or 33.01% and 23.57%, respectively, higher in SDP, while Treatment NK was 35.76% lower in SDP. The effects of Treatments NP and NK were related to the abundance of nirS gene, and the changes in content of soil available P (AP) and N:P ratio, while that of Treatment PK was associated with the community composition of nirS-type denitrifiers (Azospira sp. NC3H-14). In the upland field, compared with Treatment NPK, Treatment NP, NK and P was 13.94%, 26.51%, and 25.41%, respectively, higher in SDP. The effects of Treatments NK and P were significantly related to the abundance of narG gene and of nirS-type denitrifiers (Azospira sp. NC3H-14 and Ideonella sp. NC3L-43b for NK treatment; Azospira sp. NC3H-14, Rhodanobacter sp. D206a, Rubrivivax gelatinosus for P treatment). The content of amorphous iron oxide (Feo) was probably the main factor affecting the abundance of narG gene.[Conclusion] The above listed findings indicate that planting system affects the effect of fertilization on SDP. The variation of SDP in paddy field is mainly attributed to nirS-type denitrifiers, while that in upland field primarily to the abundance of functional gene and the community compostion of nirS-type denitrifiers.

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王海翠,刘兆东,李丹丹,柳开楼,黄庆海,赵炳梓,张佳宝.种植模式影响施肥导致的土壤反硝化势变化及其微生物机制[J].土壤学报,2022,59(1):242-252. DOI:10.11766/trxb202009250539 WANG Haicui, LIU Zhaodong, LI Dandan, LIU Kailou, HUANG Qinghai, ZHAO Bingzi, ZHANG Jiabao. Effect of Planting System on Fertilization-induced Variation of Soil Denitrification Potential and Its Microbial Mechanism[J]. Acta Pedologica Sinica,2022,59(1):242-252.

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  • 收稿日期:2020-09-25
  • 最后修改日期:2020-11-30
  • 录用日期:2021-02-08
  • 在线发布日期: 2021-02-09
  • 出版日期: 2022-01-11
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