首页 > 过刊浏览>2022年第59卷第5期 >1359-1368. DOI:10.11766/trxb202103150145
PDF HTML阅读 XML下载 导出引用 引用提醒
潮土长期不同施氮水平对秸秆降解及其细菌群落结构的影响
作者:
中图分类号:

S154.1

基金项目:

国家自然科学基金项目(41807017)和中国科学院战略性先导科技专项子课题(XDA24020104)资助


Influences of Long-term Different N Application Rates on Straw Decomposition and Bacterial Community Structure in a Fluvo-aquic Soil
Author:
  • LI Jingwang

    LI Jingwang

    State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;Fengqiu Agro-Ecological National Experimental Station, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;University of Chinese Academy of Sciences, Beijing 100049, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • CHEN Lin

    CHEN Lin

    State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;Fengqiu Agro-Ecological National Experimental Station, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • MA Donghao

    MA Donghao

    State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;Fengqiu Agro-Ecological National Experimental Station, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • ZHANG Congzhi

    ZHANG Congzhi

    State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;Fengqiu Agro-Ecological National Experimental Station, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • ZHANG Jiabao

    ZHANG Jiabao

    State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;Fengqiu Agro-Ecological National Experimental Station, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;University of Chinese Academy of Sciences, Beijing 100049, China
    在期刊界中查找
    在百度中查找
    在本站中查找
  • 摘要
    • | |
  • 访问统计
    • |
  • 参考文献 [32]
    • |
  • 相似文献 [20]
    • |
  • 引证文献
    • | |
  • 文章评论
    摘要:

    农田过量施氮所引发的问题已经引起广泛关注，但长期施氮后土壤无机氮水平状况及其对秸秆降解的作用尚不清楚。以中国科学院封丘农业生态实验站长期试验（2005—2018年）的潮土为研究对象，选择5个施氮水平（0（N0）、150（N1）、190（N2）、230（N3）和270（N4） kg·hm-2·a-1）土壤，开展短期（50 d）的秸秆降解-土壤培育实验。培育期间监测秸秆碳的矿化、土壤无机氮（硝态氮和铵态氮）、可溶性有机碳、微生物生物量碳的动态变化，利用高通量测序测定细菌群落结构。结果表明，长期施氮后，土壤无机氮含量和秸秆碳的矿化率随施氮水平的升高而增加。不同长期施氮水平的土壤细菌群落结构呈现显著差异。网络分析揭示：秸秆降解过程中细菌群落内部物种间的共现模式随长期施氮水平发生改变，具体体现为长期高施氮水平下细菌群落彼此间的负相关得到了加强；同时，变形菌主导地位减弱、酸杆菌主导地位增强。综上，土壤无机氮含量随着长期施氮水平的升高而增加，进而引起土壤细菌群落结构发生了改变，使得在秸秆降解过程中起到的作用不同，表现为秸秆降解随着施氮水平的增加而增加。本研究探究了长期不同施氮水平下土壤中无机氮的水平状况、秸秆降解状况以及秸秆降解过程中土壤细菌的生物特性，以期为秸秆还田和科学施氮提供一定的数据支撑和思路启示。

    Abstract:

    【Objective】The problems caused by excessive nitrogen(N) application in agriculture has been of growing concern, but the soil mineral N level and its effect on straw decomposition after long-term N application remain unclear. 【 Method】 We used a fluvo-aquic soil treated for 14 years(2005-2018) with N at the rates of 0(N0), 150(N1), 190(N2), 230(N3) and 270(N4) kg·hm-2·a-1, and conducted a 50-day laboratory incubation experiment with and without straw addition. Soil CO2 emission was determined during the incubation, soil mineral N(nitrate and ammonium nitrogen), dissolved organic carbon(DOC), microbial biomass carbon(MBC) were measure before and after the incubation, and the bacterial community structure was determined using amplicon sequencing of the 16S rRNA genes. 【 Result】 Results show that soil mineral N content and straw decomposition efficiency increased with increasing N application rates and the bacterial community composition was significantly(P<0.05) influenced by N application rates. The network analysis revealed that the co-occurrence pattern among species within the bacterial community during straw decomposition changed with the N application level, and the negative association among bacterial communities was strengthened under a high N application level. At the same time, the dominant position of Proteobacteria was weakened and that of Acidobacteria was enhanced.【 Conclusion】 The soil inorganic nitrogen content increased with the increase of long-term nitrogen application level, resulting in changing in soil bacterial community structure. Thus soil bacterial community structure of different long-term nitrogen application played different roles in the process of straw degradation, which was manifested as the increase of straw degradation with the increase of nitrogen application level. The relationship among soil mineral N content, bacterial community structure and species changed with the difference of long-term N application level. The results of this study provide guidelines for the use of straws to amend soils under N application.

    参考文献
    [1] Geisseler D, Scow K M.Long-term effects of mineral fertilizers on soil microorganisms-A review[J].Soil Biology&Biochemistry, 2014, 75:54-63.
    [2] Zhang F S, Wang J Q, Zhang W F, et al.Nutrient use efficiencies of major cereal crops in China and measures for improvement[J].Acta Pedologica Sinica, 2008, 45(5):915-924.[张福锁,王激清,张卫峰,等.中国主要粮食作物肥料利用率现状与提高途径[J].土壤学报, 2008, 45(5):915-924.]
    [3] Schroder J L, Zhang H L, Girma K, et al.Soil acidification from long-term use of nitrogen fertilizers on winter wheat[J].Soil Science Society of America Journal, 2011, 75(3):957-964.
    [4] Lu X, Mao Q, Gilliam F S, et al.Nitrogen deposition contributes to soil acidification in tropical ecosystems[J].Global Change Biology, 2014, 20(12):3790-3801.
    [5] Vance G F, David M B.Forest soil response to acid and salt additions-of sulfate[J].Soil Science, 1991, 151(4):297-305.
    [6] Oades J M.An introduction to organic matter in mineral soils[J].Minerals in Soil Environments, 1989:89-159.
    [7] Ju X T, Xing G X, Chen X P, et al.Reducing environmental risk by improving N management in intensive Chinese agricultural systems[J].Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(9):3041-3046.
    [8] Chen Q, Zhang X S, Zhang H Y, et al.Evaluation of current fertilizer practice and soil fertility in vegetable production in the Beijing region[J].Nutrient Cycling in Agroecosystems, 2004, 69(1):51-58.
    [9] Ju X T, Kou C L, Zhang F S, et al.Nitrogen balance and groundwater nitrate contamination:Comparison among three intensive cropping systems on the North China Plain[J].Environmental Pollution, 2006, 143(1):117-125.
    [10] Malińska K, Zabochnicka-Świątek M, Dach J.Effects of biochar amendment on ammonia emission during composting of sewage sludge[J].Ecological Engineering, 2014, 71:474-478.
    [11] Zhou J, Jiang X, Ma M C, et al.Effects of long-term nitrogen fertilization on soil fertility and microorganism:A review[J].Soil and Fertilizer Sciences in China, 2016(6):8-13.[周晶,姜昕,马鸣超,等.长期施氮对土壤肥力及土壤微生物的影响[J].中国土壤与肥料, 2016(6):8-13.]
    [12] Zhong Y, Liu J, Jia X Y, et al.Microbial community assembly and metabolic function during wheat straw decomposition under different nitrogen fertilization treatments[J].Biology and Fertility of Soils, 2020, 56(5):697-710.
    [13] Chen L, Zhang J B, Zhao B Z, et al.Soil fertility and its response to drying-wetting alternation as affected by nitrogen fertilization rate[J].Acta Pedologica Sinica, 2013, 50(4):675-683.[陈林,张佳宝,赵炳梓,等.不同施氮水平下土壤的生化性质对干湿交替的响应[J].土壤学报, 2013, 50(4):675-683.]
    [14] Chen L, Zhang J B, Zhao B Z, et al.Dynamics of nitrogen in topsoil related to integrated water and nitrogen management[J].Acta Pedologica Sinica, 2013, 50(3):459-468.[陈林,张佳宝,赵炳梓,等.不同水氮耦合管理下耕层土壤的氮动态[J].土壤学报, 2013, 50(3):459-468.]
    [15] Huang P, Zhang J B, Zhu A N, et al.Nitrate accumulation and leaching potential reduced by coupled water and nitrogen management in the Huang-Huai-Hai Plain[J].Science of the Total Environment, 2018, 610/611:1020-1028.
    [16] Huang P, Zhang J B, Zhu A N, et al.Coupled water and nitrogen (N) management as a key strategy for the mitigation of gaseous N losses in the Huang-Huai-Hai Plain[J].Biology and Fertility of Soils, 2015, 51(3):333-342.
    [17] Zhang J, Huang J S, Liu J, et al.Carbon dioxide emissions and organic carbon contents of fluvo-aquic soil as influenced by straw and lignin and their biochars[J].Journal of Agro-Environment Science, 2015, 34(2):401-408.[张杰,黄金生,刘佳,等.秸秆、木质素及其生物炭对潮土CO2释放及有机碳含量的影响[J].农业环境科学学报, 2015, 34(2):401-408.]
    [18] Dalal R C, Wang W J, Robertson G P, et al.Nitrous oxide emission from Australian agricultural lands and mitigation options:A review[J].Soil Research, 2003, 41(2):165.
    [19] Galloway J N, Townsend A R, Erisman J W, et al.Transformation of the nitrogen cycle:Recent trends, questions, and potential solutions[J].Science, 2008, 320(5878):889-892.
    [20] Wang J B, Zhu T C, Ni H W, et al.Effects of elevated CO2 and nitrogen deposition on ecosystem carbon fluxes on the Sanjiang plain wetland in Northeast China[J].PLoS One, 2013, 8(6):e66563.
    [21] Chen L, Zhang J B, Zhao B Z, et al.Carbon mineralization and microbial attributes in straw-amended soils as affected by moisture levels[J].Pedosphere, 2014, 24(2):167-177.
    [22] Milner P, Ralevic V, Hopwood A M, et al.Ultrastructural localisation of substance P and choline acetyltransferase in endothelial cells of rat coronary artery and release of substance P and acetylcholine during hypoxia[J].Experientia, 1989, 45(2):121-125.
    [23] Banerjee S, Kirkby C A, Schmutter D, et al.Network analysis reveals functional redundancy and keystone taxa amongst bacterial and fungal communities during organic matter decomposition in an arable soil[J].Soil Biology&Biochemistry, 2016, 97:188-198.
    [24] Jackson L E.Ecology in Agriculture[M].Academic Press, 1997.
    [25] Campbell B J, Polson S W, Hanson T E, et al.The effect of nutrient deposition on bacterial communities in Arctic tundra soil[J].Environmental Microbiology, 2010, 12(7):1842-1854.
    [26] Fierer N, Lauber C L, Ramirez K S, et al.Comparative metagenomic, phylogenetic and physiological analyses of soil microbial communities across nitrogen gradients[J].The ISME Journal, 2012, 6(5):1007-1017.
    [27] Ramirez K S, Craine J M, Fierer N.Consistent effects of nitrogen amendments on soil microbial communities and processes across biomes[J].Global Change Biology, 2012, 18(6):1918-1927.
    [28] Zhang X F, Xu S J, Li C M, et al.The soil carbon/nitrogen ratio and moisture affect microbial community structures in alkaline permafrost-affected soils with different vegetation types on the Tibetan Plateau[J].Research in Microbiology, 2014, 165(2):128-139.
    [29] Livesley S J, Ossola A, Threlfall C G, et al.Soil carbon and carbon/nitrogen ratio change under tree canopy, tall grass, and turf grass areas of urban green space[J].Journal of Environmental Quality, 2016, 45(1):215-223.
    [30] Blagodatskaya Е, Kuzyakov Y.Mechanisms of real and apparent priming effects and their dependence on soil microbial biomass and community structure:Critical review[J].Biology and Fertility of Soils, 2008, 45(2):115-131.
    [31] Mau R L, Liu C M, Aziz M, et al.Linking soil bacterial biodiversity and soil carbon stability[J].The ISME Journal, 2015, 9(6):1477-1480.
    [32] Bissett A, Brown M V, Siciliano S D, et al.Microbial community responses to anthropogenically induced environmental change:Towards a systems approach[J].Ecology Letters, 2013, 16:128-139.
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

李敬王,陈林,马东豪,张丛志,张佳宝.潮土长期不同施氮水平对秸秆降解及其细菌群落结构的影响[J].土壤学报,2022,59(5):1359-1368. DOI:10.11766/trxb202103150145 LI Jingwang, CHEN Lin, MA Donghao, ZHANG Congzhi, ZHANG Jiabao. Influences of Long-term Different N Application Rates on Straw Decomposition and Bacterial Community Structure in a Fluvo-aquic Soil[J]. Acta Pedologica Sinica,2022,59(5):1359-1368.

复制
分享
文章指标
  • 点击次数:633
  • 下载次数: 1723
  • HTML阅读次数: 1688
  • 引用次数: 0
历史
  • 收稿日期:2021-03-15
  • 最后修改日期:2021-06-02
  • 在线发布日期: 2022-08-16
文章二维码
今日访问量：42 您是第8261613 位访问者
通讯地址：江苏省南京市江宁区麒麟街道创优路298号 邮政编码：211135 电话：025-86881237
网址：http://pedologica.issas.ac.cn/ E-mail：actapedo@issas.ac.cn
版权所有：《土壤学报》编辑部 技术支持：北京勤云科技发展有限公司 ICP:05004320号-1