Studies of the root eaudates of wheat shoots on the growing amount and denitrification activity of the dominant denitrifiers species were conducted by the sterile aquicultural experiments.The results indicated that the rhizosphere effects of the two different genotype wheats in the denitrifiers are significant.The total amount of the bacteria are larger and the activity of denitrification are more intensive in the rhizosphere of Zhenyin No.l than those in the rhizosphere of Baofen No.7228. The components of amino acid of the root esudates of Zhenyin No.l are similar to those of Baofen No.7228,but the quantity of amino acid,the former is larger. The Table of resistance to streptomycin on the denitrifiers has no effect on the activity of denitrification.