This paper deals with the interrelations and interactions of mineral nutritive elements in the soils and flue-cured tobacco system. Samples come from five typical tobacco-growing soils-plateau red soil, red calcareous soil, alluvial red soil, yellow soil and purple soil, and three varieties of tobacco-K326, Hongda and Nc82, at six growing stages. The concentrations of K, P, S, Ca, Mg, Fe, Mn, Zn, Cu and B in the leaves and the nutrient contents extracted by AB/D multiple-elements extraction procedure, and pH content of organic matter and hydrolyzable nitrogen within the root zone soil were investigated. Principal component analysis was performed using Sinus chemometrics software. The results indicated that: i) the change in concentration of nutrients in the leaves follows a regular pattern, and the variations in chemical properties of the root zone soil are more than that between the different varieties. ii) The nutrient concentrations in the leaves are dilution effect for K, P, B, Cu, and accumulation effect for Ca iii) there is a mutual synergism among K, P, Cu and B, and an antagonism of Ca to the others in the flue-cured tobacco nutrition. iv) The key to improvement of nutrition, yield and quality of flue-cured tobacco lies in increasing organic matter content in soil and making use of the synergism of K, P, Cu and B to counteract antagonism of Ca to K, P, Cu and B.