The available S content,as measured with Ca(H₂PO₄)₂ extraction and ICP-AES,in topsoils (0~20cm)of mulberry gardens in the area of the middle and lower reaches of the Yangtze River was found to be 2.51~87.73mg kg^-1)(Mean SE=18.34±15.25mg kg^-1 ).About 55.77% of the mulberry garden topsoils in six provinces of Jiangxi,Anhui,Hubai,Hunan,Jiangsu and Fujian have an available sulfur content below the critical value.The available sulfur content in the mulberry garden topsoil was commonly found to be lower that of the subsoil.Significant two-power function correlation was found between the available sulfur content in soil of mulberry gardens and the total sulfur content of mulberry leaves(Y=-0.001x²+0.017 9x+1.791 9,R²=8.1773^**,N=52).The curvilinear correlation(two-power function relationship)between sulfur contents in mul-berry leaves and cocoon shel was significant(Y=-0.694 2x²+2.996x-1.542 3,R²=0.210 1,n=26).Sulfur concentration in cocoon shel,pupa and silkworm excrement from high-yielding mulberry gardens was higher than that from low-yielding mulberry gardens,respectively.