Two clay samples from B horizon in Alfisols developed from granite and distributed in subtropical zones, covering 30~32 north latitude were treated with DCB solution and 1/3 mol L^-1 Na-citrate solution after DCB solut ion treatment respectively The X-ray diffractometry patterns of the clay samples before and after the treatments show that: there were hydroxy materials in interlayer spaces between expansible silicate layers of the soil clay tested They could be extracted by DCB Na-ci-trate solution and mainly derived from vermiculite or some of them also could be derived from smectite.The differential FTIR spectra of the DCB Na-citrate solution treated samples show the presence of hydroxy-aluminosilicate(HAS), hydroxy-Al interlayers.These results provide evidence that HAS occurs in the interlayer spaces of expansible minerals in Afisol in central China.