There are two theories for cation-EDTA uptake: One suggested that meta-l EDTA complex formed in the soil could increase metal solubility, and promote diffusion, and, hence, elevate potential uptake. An-other theory suggested the complexation of metals with EDTA reduced the activities of free ions in the soil solution, and, therefore, decreased uptake. Pot experiment was conducted to study the role of EDTA in cadmium absorption by Indian Mustard (Brassica juncea). H₂O and NH₄NO₃ extractable Cd increased sharply in soils receiving EDTA treatment. Cadmium accumulation in the roots of B. juncea decreased significantly after EDTA application. However, Cd in shoots increased significantly when Cd added was over 130 mg kg^-1 in soils, and Cd toxicity was observed. The results suggested that Cd concentration in-creased in soil solution after addition of EDTA, which resulted in increasing absorption and translocation when plant was suffering from toxicity.