On basis of achievements of related researches, this paper studies regional ecological safety systematically from the angle of theory, technology and methodology, practical application, etc. The study covers mainly: (1) set-up of a theoretic framework for assessment of regional ecological safety, which is of explorative significance theoretically, and can be used in practice as a theoretic basis for assessment of ecological safety and a methodological basis for management of ecological environment;(2) with the application of model's and remote sensing technology, realization of extraction of information of multi-characteristic type factors and the transformation from pixellevel micro-assessment value to small basin-level meso-assessment results in as sessing regional ecological safety;(3) the assessment results. by GIS technology though mapping, expressing objectively spatial characteristics of the assessment and ensuring practicality of the research findings.