An improved method was used to extract soil microbial DNA by indigenous silica gel instead of imported glass beads. A new degenerate PCR-primer pair P1/P2 was designed to amplify directly xylanase gene fragments from soil microbial DNA. PCR products were ligated with pMD18T vector. Transformants were got by transforming ligation product into E. coli. Ten different recombinants that were confirmed by RFLP were sequenced. The sequence analysis shows that all of the ten fragments encode partial xylanase. The BLAST of deduced amino acid of fragments show that eight out of the ten were high in sequence homology with xylanase from Actinomycetes, two with Pseudomonas. Asn27 was found in all partial xylanase based on the alignment of deduced amino acid sequences, which means that all xylanase fragments were alkali-tolerant. Phylogenetic tree shows that all xylanase fragments were positive and over 70% of similarity.