In a pot experiment, it was found that host infection rates, and activities of hyphal SDH (succinate dehydrogenase) and ALP (alkaline phosphatase) enzymes of Glomus intraradices (G.i), Acaulospora laevis (A.l) and G.i + A.l were significantly enhanced by hesperitin.After treatment with 15 nmol L^-1, 150 nmol L^-1, and 1.5μ mol L^-1 hesperitin, respectively, samples of maize and cotton roots were collected for test at the 6th week.Results of the tests showed that with the maize roots, the G.i infection rates were 76.2%, 84.5% and 75.8% (the control was 45.9%), hyphal SDH activity of G.i were 68.4%, 75.9% and 67.4% (the control was 40.7%), and hyphal ALP activity of G.i were 45.8%, 51.4% and 45.1% (the control was 27.1%), respectively.And with the cotton roots, the G.i infection rates were 85.2%, 88.9% and 83.8% (the control was 59.8%), hyphal SDH activity were 76.8%, 81.2% and 75.0% (the control was 53.1%), hyphal ALP activity were 51.2%, 53.7% and 49.4% (the control was 35.2%), respectively.And hesperitin could increase significantly total amount of hyphae of AM fungus in the soil.The biomass of maize and cotton could also be affected by hesperitin.