A three-year (2005～2007) experiment was conducted in paddy fields under a double rice cropping system to study effects of continuous no-tillage and cast-transplanting on soil physical, chemical and biological properties. Results show that no-tillage treatment for one year（two crops）improved soil physical properties, but no-tillage treatment for three years (six crops), worsened soil physical properties. However, the effect of no-tillage treatment for two years varied. In the soil incorporated with milk vetch and rice straw, it decreased soil density of the cultivated horizon, but increased total porosity and non-capillary porosity therein, and helped nutrient enrichment in the surface soil layer. Soil analysis showed that the no-tillage field was less than the control, plowed field in total amount of three groups of soil microbes. However, in no-tillage field, the amount of soil bacteria increased, while that of soil actinomycosis and fungi reduced, and urease activity in surface layer soil increased, while catalase and peroxidase activity reduced. Significantly positive correlations were observed of soil organic matter and total N with urease, catalase and polyphenol oxidase activity and, significantly positive correlations of soil total N and available K with peroxidase activity and. The findings provide a valuable reference for popularization and application of the technology of no-tillage and cast-transplantation of rice in the future.