A field experiment, designed to have 5 N levels, i.e. 0, 100, 150, 200 and 250 kg hm^-2, was conducted in 2005 to study effects of N application rate on uptake, accumulation, and translocation of N by rice germ plasm 4007 high in nitrogen use efficiency (NUE), and yield of the crop, with a native rice cultivar (Wuyunjing15, WJ15) as CK. Results show that increasing N application significantly stimulated N accumulation in rice plants at all growth stages. The highest was found during the stages between mid-tillering and jointing, being 32.7%～41.6% of the total of the whole growth period. When N application rate increased from 0 up to 250 kg hm^-2, N translocation was also enhanced, rising from N 72.0 kg hm^-2 to 104 kg hm^-2 for 4007, and N 57.0 kg hm^-2 to 96.5 kg hm^-2 for WJ15, but their nitrogen translocation rate declined from 66.2% to 51.3% and from 57.1% to 46.8% for 4007 and WJ15, respectively. Of the N in grains, 65.3%～87.6% came from vegetative organs of the plant, and only 12.4%～34.7% was absorbed from soil at the reproductive stage. Thanks to the higher harvest index (HI) and nitrogen harvest index (NHI), 4007 showed higher mean nitrogen apparent recovery efficiency (RE_N) and nitrogen agronomic efficiency (AE_N), about 24.5% and 95.6% higher than WJ15 did, respectively. The experiment suggests that the optimal N application rate is 150 kg hm^-2 and 200 kg hm^-2, respectively, for 4007 and WJ15 to maintain a better grain yield and a better nitrogen use efficiency.