Effects of repeated application of fungicide carbendazim at a rate of 0.94, 1.88, 4.70 kg hm^-2 calculated by active ingredient (a.i.) on diversity of soil microbial communities were studied using BIOLOG and its degradation dynamics in soil was investigated under field conditions. The degradation rate of carbendazim after the first application in the soil was 0.11, 0.25 and 0.74 mg kg^-1d^-1, in treatments of 0.94, 1.88 and 4.70 kg hm^-2 calculated by a.i., respectively, and rose to 0.8, 0.39 and 1.00 mg kg^-1d^-1, respectively after the fourth application, showing a significant trend of the degradation rate rising with the application frequency. A significant inhibitory effect of carbendazim on average well color development (AWCD) was observed after the first application. The AWCD values then increased slightly with the application frequency Shannon, Simpson and Shannon indexes of the soil microbial communities in the soil were also inhibited by the first application, which suggested that the richness, evenness and dominant character of soil communities in the treated soils were altered by the repeated application of carbendazim. However, after the second application, soil microorganisms gradually adapted to carbendazim and recovered to the level in the control without carbendazim treatment.