Mudflat soils different in salt concentration were collected from Dafeng, Jiangsu Province, China. The soils were tested for culturability of bacteria, using different culture schemes and the technology of DGGE (denaturing gradient gel electrophoresis). Results show that natural sea water was necessary as a media supplement in culturing bacteria in all soils. For cAMP did not show much improvement in bacterial culturability. Concentration of the medium was a key factor affecting the culturability. Prolonged incubation up to 25 days increased the number of colony-forming units on the plate. Liquid media were better than solid ones in terms of culturing maximal number of bacteria. By the most probable number method, it was noted that tubes with limpid solution and some mucous precipitation, which used to be overlooked as “negative”, should be taken as “positive”. Microscopic and sequence examination revealed that the mucous precipitation consisted mainly of small short rodshaped bacteria (0.6×1.2~2.6 μm), highly similar to Bacillus sp. An optimal culture scheme for the mudflat soils is proposed to have liquid culture medium consisting of 1/10 nutrient broth and 100% natural sea water, and 25-day incubation. Further study should be conducted to optimize carbon compounds in the media.