Variation of soil zinc in form in calcareous soil, a potentially zinc-deficient soil, was analyzed after application of zinc fertilizer in the soil with a view to investigating availability and aftereffect of Zn fertilizer. Results show that wheat did not improve much its yield, but did Zn concentration in grains; and the effect varied from genotype to genotype. In the soil Zn appeared mainly in the following forms; mineral Zn accounting for 91.5%-97.6% of total Zn, organic-matter-loosely-bonded Zn for 1.34%-5.53% and carbonate-bonded Zn for 0.47%-1.55%. Application of Zn fertilizer increased various fractions of Zn in the soil, such as exchangeable Zn, organic-matter-loosely-bonded Zn, and carbonate-bonded Zn and the manganese oxides bounded Zn, and most of the applied zinc transformed into mineral form. Wheat plantation helped Zn in the soil turn into available form. Application of nitrogen fertilizer enhanced Zn uptake by wheat, and expanded the fraction of mineral Zn. Principal component analysis reveals that the fractions of exchangeable Zn, organic-matter-loosely-bonded Zn, and carbonate-bonded Zn are to some extent indicators of Zn availability.In calcareous soil carbonate-bounded Zn and organic-matter-loosely-bonded Zn account make up a considerable portion of total Zn.Therefore, to enlarge the pools of the two fractions of Zn is key to management of soil Zn nutrient in calcareous soil.